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首页> 外文期刊>Environmental toxicology and chemistry >PYRENE METABOLISM IN THE SPRINGTAIL ORCHESELLA CINCTA L. (COLLEMBOLA, ENTOMOBRYIDAE)
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PYRENE METABOLISM IN THE SPRINGTAIL ORCHESELLA CINCTA L. (COLLEMBOLA, ENTOMOBRYIDAE)

机译：金枪鱼Orchessella cincta L.（Collembola，昆虫科）中的Y代谢

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摘要

A novel, solvent-free spiking method was used to contaminate foodstuff (Desmococcus spp. algae) with pyrene for bioassays with the springtail Orchesella cincta L. (Collembola, Entomobryidae). The primary metabolite 1-hydroxypyrene and two conjugates (pyrene-1-glucoside and one tentatively identified as pyrene-1-glucosidemalonate) were quantified in the insects by high-performance liquid chromatography (HPLC) with fluorescence detection. Individuals from an unpolluted site (SUS population) were capable of rapid metabolism of pyrene, reaching an apparent steady state after 24 h with a composite assimilation/transformation rate of approximately 25 pmol pyrene · g~(-1) fresh weight · h~(-1) at an exposure level of approximately 18 μg pyrene · g~(-1) dry weight algae. A cadmium (Cd)-tolerant population (TOL), genetically distinct from SUS, was exposed in parallel and the populations' pyrene metabolism compared in order to gain insight into any potential cost of Cd-tolerance. The TOL animals assimilated and/or performed phase 1 transformation of pyrene approximately twice as fast as SUS (～56 pmol · g~(-1) · h~(-1)), but there was no significant difference in the elimination rate constants between SUS and TOL animals. Attributing this difference to any specific cost of Cd-tolerance requires more work and may be coincidental to metal tolerance, but pyrene metabolism in these distinct genotypes, subjected to different selection pressures, was nevertheless significantly different.
机译：一种新颖的，无溶剂的加标方法被用于用food污染食品（Desmococcus spp。藻类），用于与长尾Orchesella cincta L.（Collembola，Entomobryidae）进行生物测定。通过高效液相色谱（HPLC）和荧光检测对昆虫体内的主要代谢物1-羟基py和两种结合物（py-1-葡萄糖苷和一种初步鉴定为pyr-1-葡萄糖苷丙二酸酯）进行定量。来自无污染地点（SUS种群）的个体能够快速metabolism的代谢，在24 h后达到表观稳定状态，复合同化/转化速率约为25 pmol··g〜（-1）鲜重·h〜（ -1）在约18μg··g〜（-1）干重藻类的暴露水平下。遗传上不同于SUS的耐受镉（Cd）的种群（TOL）平行暴露，并对种群的pyr代谢进行比较，以便深入了解耐受Cd的任何潜在成本。 TOL动物吸收和/或进行1的1期转化的速度约为SUS（〜56 pmol·g〜（-1）·h〜（-1））的两倍，但消除速率常数没有显着差异在SUS和TOL动物之间。将此差异归因于Cd耐受性的任何特定成本，这需要更多的工作，并且可能与金属耐受性相吻合，但是这些不同基因型在不同的选择压力下的pyr代谢却存在显着差异。

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