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首页> 外文期刊>Environmental toxicology and chemistry >INTEGRATING ENZYMATIC RESPONSES TO ORGANIC CHEMICAL EXPOSURE WITH TOTAL OXYRADICAL ABSORBING CAPACITY AND DNA DAMAGE IN THE EUROPEAN EEL ANGUILLA ANGUILLA
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INTEGRATING ENZYMATIC RESPONSES TO ORGANIC CHEMICAL EXPOSURE WITH TOTAL OXYRADICAL ABSORBING CAPACITY AND DNA DAMAGE IN THE EUROPEAN EEL ANGUILLA ANGUILLA

机译：整合酶对有机化学暴露的反应与欧洲鳗鱼的总吸氧能力和DNA损伤

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In this work, susceptibility to oxidative stress was analyzed under laboratory conditions in the European eel Anguilla anguilla. Eels were treated with increasing concentrations of benchmark environmental pollutants, namely, benzo[a]pyrene ([BaP], at 0, 0.1, 1, 10, and 50 mg/kg), β-naphthoflavone ([BNF], at 0, 0.1, 1, 10, and 50 mg/kg), Arochlor~(~R) 1254 (at 0, 0.1, 1, 10, and 50 mg/kg), and 2,3,7,8-tetrachlorodibenzo p-dioxin ([TCDDJ, at 0, 0.01, 0.1, 1, and 2 μg/kg). The integral relationships were analyzed between induction of ethoxyresorufin O-deethylase (EROD) activity, its involvement in perturbing oxyradical metabolism, and the role of cytochrome P450 and/or oxidative stress in mediating genotoxic effects. To reveal whether the oxidative status in exposed organisms was altered as a result of chemical exposure, measurements of the main endogenous antioxidant defenses were integrated with the measurement of total oxyradical scavenging capacity (TOSC) toward peroxyl radicals and hydroxyl radicals (·OH). This approach permits discriminating the resistance of a tissue toward different forms of oxyradicals, thereby indicating a differential role for specific reactive oxygen species (ROS) in perturbing the balance between prooxidant and antioxidant mechanisms. All the analyzed chemicals promoted EROD induction (reflective of CYP1A) and altered either the levels or the activities of the antioxidants studied, which might be anticipated to exert alterations in oxyradical metabolism. Analysis of TOSC suggested the prevalence of metabolic oxidative pathways leading to the more reactive ·OH on exposure to the chemicals studied. Of these chemicals, enhanced EROD activity correlated with genotoxic damage only in the cases of the nonhalogenated hydrocarbons BaP and BNF. The highest degree of genotoxic damage was consistently observed in organisms in which the capacity to absorb or scavenge ·OH was lowest. These data suggest a general relationship between oxidative stress and loss of DNA integrity in juvenile eels exposed to the chemicals studied herein.
机译：在这项工作中，在欧洲鳗鳗的实验室条件下分析了对氧化应激的敏感性。对鳗鱼用浓度不断增加的基准环境污染物进行处理，即苯并[a] py（[BaP]，分别为0、0.1、1、10和50 mg / kg），β-萘黄酮（[BNF]，为0， 0.1、1、10和50 mg / kg），Arochlor®（〜R）1254（0、0.1、1、10和50 mg / kg）和2,3,7,8-四氯二苯并二恶英（[TCDDJ，分别为0、0.01、0.1、1和2μg/ kg）。分析了乙氧基间苯二酚O-脱乙基酶（EROD）活性的诱导，其参与扰动氧自由基代谢以及细胞色素P450和/或氧化应激在介导基因毒性作用中的作用之间的整体关系。为了揭示暴露的生物体中的氧化状态是否由于化学暴露而改变，将主要的内源性抗氧化剂防御措施与总过氧自由基清除能力（TOSC）对过氧自由基和羟自由基（·OH）的测量结合在一起。这种方法可以区分组织对不同形式的氧自由基的抵抗力，从而表明特定的活性氧（ROS）在扰动前氧化剂和抗氧化剂机制之间的平衡方面起着不同的作用。所有分析过的化学物质均能促进EROD诱导（反映CYP1A）并改变所研究抗氧化剂的水平或活性，这可能预期会导致氧自由基代谢的改变。 TOSC的分析表明，代谢氧化途径的普遍存在导致暴露于所研究化学物质时反应性更高的·OH。在这些化学物质中，仅在非卤代烃BaP和BNF的情况下，增强的EROD活性与遗传毒性损害相关。在吸收或清除·OH能力最低的生物中始终观察到最高程度的遗传毒性破坏。这些数据表明在暴露于本文研究的化学物质的幼鳗中氧化应激与DNA完整性丧失之间的一般关系。

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