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首页> 外文期刊>Environmental toxicology and chemistry >PHOSPHOLIPASE A2 ACTIVITY IN THREE SPECIES OF LITTORAL FRESHWATER ROTIFERS EXPOSED TO SEVERAL TOXICANTS
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PHOSPHOLIPASE A2 ACTIVITY IN THREE SPECIES OF LITTORAL FRESHWATER ROTIFERS EXPOSED TO SEVERAL TOXICANTS

机译：暴露于几种毒素的三种滨海淡水轮虫中磷脂酶A2的活性

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摘要

We analyzed three species of Lecane, a littoral rotifer, for susceptibility to six metals and four organic toxicants using a fluorometric assay based on inhibition of activity of the enzyme phospholipase A2. The metallic toxicants that we tested included Cd, Cr, Cu, Pb, Hg (as HgCl_2), and Ti; the organic toxicants included benzene, ethyl acetate, toluene, and vinyl acetate. The three species differed greatly with respect to their susceptibility to the various toxicants. Lecane quadridentata, for example, was particularly sensitive to the four organic compounds (median effective concentration values [EC50] ranged from 6.6 X 10~(-4)-0.987 mg/L). Lecane luna, in contrast, seemed particularly sensitive to metals (EC50 values ranged from 2 X 10~(-6)-1.92 mg/L). Lecane hamata was relatively insensitive to organic solvents (EC50 values ranged from 4.25-126.5 mg/L).
机译：我们基于荧光酶A2活性的抑制作用，通过荧光分析法分析了三种Lecane（一种近缘轮虫）对六种金属和四种有机毒物的敏感性。我们测试的金属毒物包括Cd，Cr，Cu，Pb，Hg（以HgCl_2的形式）和Ti。有机毒物包括苯，乙酸乙酯，甲苯和乙酸乙烯酯。这三种物种对各种毒物的敏感性差异很大。例如，Lecane quadridentata对四种有机化合物特别敏感（中位有效浓度值[EC50]为6.6 X 10〜（-4）-0.987 mg / L）。相反，Lecane luna似乎对金属特别敏感（EC50值范围为2 X 10〜（-6）-1.92 mg / L）。 Lecane hamata对有机溶剂相对不敏感（EC50值范围为4.25-126.5 mg / L）。

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