Monoclonal antibody enzyme-liked immunosorbent assay to quantify vitellogenin form studies on environmental estrogens in the rainbow trout(oncorhynchus mykiss)

Charles R.Tyler; Ronny van Aerle; Mona V.Nilsen

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Monoclonal antibody enzyme-liked immunosorbent assay to quantify vitellogenin form studies on environmental estrogens in the rainbow trout(oncorhynchus mykiss)

机译：单克隆抗体酶样免疫吸附法定量分析虹鳟鱼中环境雌激素的卵黄蛋白原形式

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Vitellogenin(VTG) induction has proved to be a valuable biomarker for assessing exposure to environmental estrogens in fish. The widespread use of VTG in this regard has lead to the need for standardized assays to quantify VTG, and monoclonal antibodies have the potential to help accomplish this, A VTG enzyme-linked immunosorbent assay(ELISA) was developed using a monoclonal antibody prepared against Atlantic salmon(Salmo salar) VTG(Mab BN-5) and its ability to quantify VTG in the rainbow trout(Oncorhynchus mykiss) compared with a rainbow trout vitellogenin(rt-VTG) ELISA that employed homologous polyclonal antibodies (Pab).

机译：卵黄蛋白原（VTG）诱导已被证明是评估鱼类对环境雌激素暴露的有价值的生物标志物。 VTG在这方面的广泛使用导致需要定量化VTG的标准化测定方法，单克隆抗体具有帮助实现这一目标的潜力，使用针对大西洋的单克隆抗体开发了VTG酶联免疫吸附测定（ELISA）鲑鱼（Salmo salar）VTG（Mab BN-5）及其对虹鳟鱼（Oncorhynchus mykiss）中VTG定量的能力与采用同源多克隆抗体（Pab）的虹鳟鱼卵黄蛋白原（rt-VTG）ELISA相比。

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《Environmental toxicology and chemistry》 |2002年第1期|p.47-54|共8页
作者
Charles R.Tyler; Ronny van Aerle; Mona V.Nilsen;
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收录信息美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类环境卫生、环境医学;毒物学（毒理学）;化学工业废物处理与综合利用;
关键词
Vitellogenin; Biomarker; Monoclonal antibodies; Estrogenic;

机译：卵黄蛋白原;生物标志物单克隆抗体;雌激素;

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1. Characterization of a monoclonal antibody against the 47-kDa antigen of Cryptobia salmositica Katz and its use in an antigen-capture enzyme-linked immunosorbent assay for detection of parasite antigen in infected rainbow trout, Oncorhynchus mykiss (W [J] . Verity CK, Woo PTK Journal of Fish Diseases . 1996,第1期

机译：一种针对美隐隐孢子虫（Cryptobia salmositica Katz）47 kDa抗原的单克隆抗体的表征及其在抗原捕获酶联免疫吸附测定中的应用，用于检测被感染的虹鳟鱼（Oncorhynchus mykiss）的寄生虫抗原（W
2. Characterization of a monoclonal antibody against the 47-kDa antigen of Cryptobia salmositica Katz and its use in an antigen-capture enzyme-linked immunosorbent assay for detection of parasite antigen in infected rainbow trout, Oncorhynchus mykiss (W [J] . Verity CK, Woo PTK Journal of Fish Diseases . 1996,第1期

机译：一种针对美隐隐孢子虫（Cryptobia salmositica Katz）47 kDa抗原的单克隆抗体的表征及其在抗原捕获酶联免疫吸附测定中的应用，用于检测被感染的虹鳟鱼（Oncorhynchus mykiss）的寄生虫抗原（W
3. A real-time polymerase chain reaction assay for identification and quantification of Flavobacterium psychrophilum and application to disease resistance studies in selectively bred rainbow trout Oncorhynchus mykiss [J] . Marancik D.P., Wiens G.D. FEMS Microbiology Letters . 2013,第2期

机译：实时聚合酶链反应测定法可鉴定和定量黄杆菌，并将其应用于选择性繁殖虹鳟Oncorhynchus mykiss的抗病性研究
4. Comparison of Biochemical Parameters of Rainbow Trout (Oncorhynchus mykiss) Reared in Two Different Trout Farms' [C] . Tayfun KARATAS International Conference on Advances in Natural and Applied Sciences . 2016

机译：彩虹鳟鱼生化参数（Oncorhynchus mykiss）饲养两种不同的鳟鱼养殖场
5. Quantifying the Effects of Non-native Rainbow Trout (Oncorhynchus mykiss) Hybridization and Environmental Conditions on Fitness-related Traits of Westslope Cutthroat Trout (Oncorhynchus clarkii lewisi) [D] . Strait, Jeffrey Thomas. 2020

机译：量化非本土彩虹鳟鱼（Oncorhynchus Mykiss）杂交和环境条件对Westslope Cutthroat Trout（Oncorhynchus Clarkii Lewisi）的健身相关性状
6. Efficiency of advanced wastewater treatment technologies for the reduction of hormonal activity in effluents and connected surface water bodies by means of vitellogenin analyses in rainbow trout (Oncorhynchus mykiss) and brown trout (Salmo trutta f. fario) [O] . Anja Henneberg, Rita Triebskorn -1

机译：借助于虹鳟鱼（Oncorhynchus mykiss）和褐鳟鱼（Salmo trutta f。fario）中的卵黄蛋白原分析先进的废水处理技术可有效降低废水和相连地表水体的激素活性。
7. Vitellogenin Incorporation into Oocytes of Rainbow Trout, Oncorhynchus mykiss, in Vitro: Effect of Hormones on Denuded Oocytes. (vitellogenin/oocyte growth/insulin/thyroxine/rainbow trout) [O] . Naoki Shibata, Michiyasu Yoshikuni, Yoshitaka Nagahama 1993

机译：Vitellogenin掺入虹鳟鱼，oncorhynchus mykiss，体外造成的卵母细胞：激素对裸露的卵母细胞的影响。（vitellogenin /卵母细胞生长/胰岛素/甲状腺素/虹鳟）
8. Support: Inhibition of Testicular Growth in Rainbow Trout (oncorhynchus Mykiss) Exposed to Estrogenic Alkylphenolic Chemicals, with Cover Letter dated 12/19/95. [R] . 1996

机译：支持：抑制暴露于雌激素烷基酚类化学物质的虹鳟（oncorhynchus mykiss）中的睾丸生长，其封面信件日期为12/19/95。

Monoclonal antibody enzyme-liked immunosorbent assay to quantify vitellogenin form studies on environmental estrogens in the rainbow trout(oncorhynchus mykiss)

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