Methyltransferase: an enzyme assay for microbial methylmercury formation in acidic soils and sediments

Steven D.Siciliano; David R.S.Lean

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Methyltransferase: an enzyme assay for microbial methylmercury formation in acidic soils and sediments

机译：甲基转移酶：一种用于测定酸性土壤和沉积物中微生物甲基汞形成的酶法

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Methylmercury (MeHg) is a highly toxic form of mercury that bioaccumulates in aquatic food chains. However, methods to reliably identify sites of MeHg formation or to quantify MeHg production require the use of isotopic tracers, which limits their use. In this paper, a method is presented to quantify the methylation of mercury by a methyltransferase pathway. This methyltrans- ferase pathway is one of the biochemical pathways responsible for biological mercury methylation. Protein is extracted from environmental samples, and mercury methyltransferase(HgMT)activity of soil extracts is calculated by assessing increases in methyltransferase activity induced by Hg addition.

机译：甲基汞（MeHg）是汞的一种剧毒形式，会在水生食物链中生物蓄积。但是，可靠地识别MeHg形成位点或量化MeHg产生的方法需要使用同位素示踪剂，这限制了它们的使用。本文提出了一种通过甲基转移酶途径量化汞甲基化的方法。这种甲基转移酶途径是负责生物汞甲基化的生化途径之一。从环境样品中提取蛋白质，并通过评估添加汞引起的甲基转移酶活性的增加来计算土壤提取物的汞甲基转移酶（HgMT）活性。

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来源
《Environmental toxicology and chemistry》 |2002年第6期|p.1184-1190|共7页
作者
Steven D.Siciliano; David R.S.Lean;
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收录信息美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类环境卫生、环境医学;毒物学（毒理学）;化学工业废物处理与综合利用;
关键词
Methylmercury; Methionine synthase; Wetlands; MeHg production;

机译：甲基汞蛋氨酸合酶;湿地;甲基汞生产;

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Methyltransferase: an enzyme assay for microbial methylmercury formation in acidic soils and sediments

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