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首页> 外文期刊>Environmental toxicology and chemistry >CHARACTERIZATION OF ENZYMES INVOLVED IN BIOTRANSFORMATION OF POLYCYCLIC AROMATIC HYDROCARBONS IN TERRESTRIAL ISOPODS
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CHARACTERIZATION OF ENZYMES INVOLVED IN BIOTRANSFORMATION OF POLYCYCLIC AROMATIC HYDROCARBONS IN TERRESTRIAL ISOPODS

机译:陆地同位素中多环芳烃生物转化过程中涉及的酶的表征

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摘要

Little is known about the capacity of terrestrial invertebrates to transform organic soil pollutants such as polycyclic aromatic hydrocarbons(PAHs). Studies were designed to characterize microsomal mixed function oxygenase and accompanying conjugation enzymes from the hepatopancras of terrestrial isopods Porcellio scaber and Oniscus asellus using pyrene and 1- hydorxyrene as model substrates. The hydroxylation of pyrene and the formation of pyreneglucoside and pyrenesulfate appeared to be sensitive measures for the activity of cytochrome P450 aryl hydrocarbon hydroxylase(AHH), uridinediphosphateglucosyl- transferase(UDPGT), and aryl sulforansferase(ST), respectively.
机译:关于陆地无脊椎动物转化有机土壤污染物(如多环芳烃(PAHs))的能力知之甚少。设计研究的目的是使用mixed和1- hy作为模型底物,表征来自陆生等足动物Porcellio scaber和Oniscus asellus的肝足an的微粒体混合功能加氧酶和伴随的共轭酶。 ene的羟基化以及of葡萄糖苷和pyr硫酸盐的形成似乎分别是对细胞色素P450芳基烃羟化酶(AHH),尿苷二磷酸葡糖基转移酶(UDPGT)和芳基磺基转移酶(ST)活性的敏感措施。

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