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首页> 外文期刊>Environmental toxicology and chemistry >KINETIC ANALYSIS OF ZINC ACCUMULATION IN THE GILLS OF JUVENILE RAINBOW TROUT: EFFECTS OF ZINC ACCLIMATION AND IMPLICATIONS FOR BIOTIC LIGAND MODELING
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KINETIC ANALYSIS OF ZINC ACCUMULATION IN THE GILLS OF JUVENILE RAINBOW TROUT: EFFECTS OF ZINC ACCLIMATION AND IMPLICATIONS FOR BIOTIC LIGAND MODELING

机译：幼体虹鳟G Z中锌累积的动力学分析：锌的吸收效应及其对生物配体建模的影响

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Juvenile rainbow trout were acclimated to hard water (Ca~(2+) = l.0 mM, Mg~(2+) = 0.2mM; hardness = 120 mg CaCO_3/ L) and hard water plus 250 ug/L Zn (3.8 uM). After 30 d of exposure, there was no difference in the total Zn levels of the gills of Zn-exposed and control fish (~ 70 ug Zn/g gill). Exposure of both groups to a range of Zn concentrations (0-2,900 ug/L Zn) for up to 7 d also had no effect on the measured total Zn levels in the gills. However, using radiolabeled ~(65)Zn, measurement of new Zn appearance in the gills was possible. Trout were exposed to a range of Zn concentrations (with ~(65)Zn) and the gills were sampled at times ranging from 0.5 to 72 h. The fast turnover pool of Zn in the gills increased with increasing acute Zn exposure concentration, while the maximum size of the fast pool was about ninefold larger in Zn-acclimated fish (4.14 ug Zn/g gill) versus control fish (0.45 ug Zn/g gill). At all sampling times, gill ~(65)Zn accumulation exhibited saturation kinetics, allowing calculation of binding capacity (B_(max)) and affinity (K_d). In both control and Zn-acclimated trout, K_d decreased rapidly (affinity increased) from 0.5 to 3 h and then remained constant up to 72 h. B_max increased rapidly from 0.5 to 3 h in both groups, then the rate of increase began to subside but was still increasing from 24 to 72 h. At all times, the K_d of Zn-acclimated fish was higher (i.e., lower affinity) and B_(max) was greater than controls. The stabilized K_ds (>3 h) were approximately 280 ug/L total Zn (log K = 5.6 as Zn~(2+)) and 575 ug/L total Zn (log K = 5.3 as Z
机译：将幼年虹鳟鱼适应硬水（Ca〜（2+）= 1.0 mM，Mg〜（2+）= 0.2mM;硬度= 120 mg CaCO_3 / L）和硬水加250 ug / L Zn（3.8嗯）暴露30天后，暴露于锌的鱼和对照鱼的ill的总Zn水平没有差异（〜70 ug Zn / g ill）。两组暴露于最高浓度的Zn浓度范围（0-2,900 ug / L Zn）达7 d也对on中测得的总Zn水平没有影响。但是，使用放射性标记的〜（65）Zn可以测量the中新的Zn外观。鳟鱼暴露于一定范围的锌浓度（含〜（65）Zn）中，at的采样时间为0.5至72 h。 acute中锌的快速周转池随急性锌暴露浓度的增加而增加，而适应锌的鱼（4.14 ug Zn / g ill）中快速池的最大尺寸约大九倍，而对照鱼（0.45 ug Zn /克g）。在所有采样时间，g〜（65）Zn积累均表现出饱和动力学，从而可以计算结合能力（B_（max））和亲和力（K_d）。在对照和锌适应的鳟鱼中，K_d从0.5到3 h迅速下降（亲和力增加），然后保持恒定直到72 h。两组的B_max从0.5小时迅速增加到3小时，然后增加速率开始消退，但仍从24小时增加到72小时。在任何时候，适应锌的鱼的K_d都较高（即亲和力较低），而B_（max）则大于对照。稳定的K_ds（> 3 h）约为280 ug / L的总锌（log K = 5.6作为Zn〜（2+））和575 ug / L的总Zn（log K = 5.3作为Z

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