AN OPTIMIZED MICROTITERPLATE ASSAY TO DETECT ACETYLCHOLINESTERASE ACTIVITY IN INDIVIDUAL CHIRONOMUS RIPARIUS MEIGEN

THOMAS C. FISHER; MARK CRANE; AMANDA CALLAGHAN

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AN OPTIMIZED MICROTITERPLATE ASSAY TO DETECT ACETYLCHOLINESTERASE ACTIVITY IN INDIVIDUAL CHIRONOMUS RIPARIUS MEIGEN

机译：优化的微晶铝检测法检测个体Chironomus RipariusMégen的乙酰胆碱酯酶活性

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A sensitive microtiter plate assay to detect acetylcholinesterase activity in fourth-instar Chironomus riparius (Meigen) larvae was developed and optimized to allow the measurement of pesticide inhibition in individuals. Optimal conditions, including enzyme concentration, pH, and substrate concentration, allow the sensitive measurement of changes in activity following exposure to the organophosphate insecticide chlorpyrifos. Significant reductions in activity were observed at pesticide doses of 0.05, 0.l, l.0, and 5 mg/L following a 48-h exposure.

机译：开发并优化了一种灵敏的微量滴定板测定法，用于检测四龄拟南芥（Meirgen）幼虫的乙酰胆碱酯酶活性。最佳条件，包括酶浓度，pH和底物浓度，可以敏感地测量暴露于有机磷酸盐杀虫剂毒死rif后的活性变化。暴露48小时后，以0.05、0.1、1.0和5 mg / L的农药剂量观察到活性显着降低。

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来源
《Environmental toxicology and chemistry》 |2000年第7期|1749-1752|共4页
作者
THOMAS C. FISHER; MARK CRANE; AMANDA CALLAGHAN;
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作者单位

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收录信息美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类环境卫生、环境医学;毒物学（毒理学）;化学工业废物处理与综合利用;
关键词
Chironomus riparius; Acetylcholinesterase; Organophosphorus; Insecticides;

机译：希波罗氏菌;乙酰胆碱酯酶;有机磷;杀虫剂;

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1. Effects of imidacloprid exposure on Chironomus riparius Meigen larvae: Linking acetylcholinesterase activity to behaviour [J] . H.M.V.S. Azevedo-Pereira, M.F.L. Lemos, A.M.V.M. Soares Ecotoxicology and Environmental Safety . 2011,第5期

机译：吡虫啉暴露对河岸线虫美根幼虫的影响：将乙酰胆碱酯酶活性与行为联系起来
2. Comparison of Acetylcholinesterase and Glutathione S-Transferase Activity in Chironomus riparius Meigen Exposed to Chemical-Spiked Sediments [J] . R. Kheir, H. Ibrahim, J. Lewis, Bulletin of Environmental Contamination and Toxicology . 2001,第5期

机译：化学加标沉积物暴露于河豚气单胞菌乙酰胆碱酯酶和谷胱甘肽S-转移酶活性的比较
3. Comparison of acetylcholinesterase and glutathione S-transferase activity in Chironomus riparius Meigen exposed to chemical-spiked sediments. [J] . Kheir R, Ibrahim H, Lewis J, Bulletin of Environmental Contamination and Toxicology . 2001,第5期

机译：乙酰胆碱酯酶和谷胱甘肽S-转移酶活性的比较啶基普里斯植物暴露于化学沉积物沉积物。
4. Quantitative characterization of response behaviors and individual variation in Chironomus riparius after treatments of diazinon [C] . O. Beauchard, K. H. Choi, S. H. Lee, Ecosystems and sustainable development VII . 2009

机译：二嗪农处理后河岸罗非鱼响应行为和个体变异的定量表征
5. Towards the Optimization of the Preclinical PET Systems for Minimum Detectable Activity Performance. [D] . Bao, Qinan. 2010

机译：致力于临床前PET系统的优化，以实现最低的可检测活动性能。
6. A Smartphone Camera Colorimetric Assay of Acetylcholinesterase and Butyrylcholinesterase Activity [O] . Miroslav Pohanka, Jitka Zakova 2021

机译：乙酰胆碱酯酶和丁酰胆碱酯酶活性的智能手机相机比色测定法
7. Hypoxia and anoxia effects on alcohol dehydrogenase activity and hemoglobin content in Chironomus riparius Meigen, 1804 [O] . Valentina Grazioli, Bruno Rossaro, Paolo Parenti, 2016

机译：低氧和缺氧对摇蚊（Chironomus riparius meigen），1804年乙醇脱氢酶活性和血红蛋白含量的影响

AN OPTIMIZED MICROTITERPLATE ASSAY TO DETECT ACETYLCHOLINESTERASE ACTIVITY IN INDIVIDUAL CHIRONOMUS RIPARIUS MEIGEN

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