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首页> 外文期刊>Environmental toxicology and chemistry >TOXICITY, SILVER ACCUMULATION AND METALLOTHIONEIN INDUCTION IN FRESHWATER RAINBOW TROUT DURING EXPOSURE TO DIFFERENT SILVER SALTS
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TOXICITY, SILVER ACCUMULATION AND METALLOTHIONEIN INDUCTION IN FRESHWATER RAINBOW TROUT DURING EXPOSURE TO DIFFERENT SILVER SALTS

机译：暴露于不同银盐下的新鲜虹鳟鱼的毒性，银累积和金属硫蛋白的诱导

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Static-renewal 168-h toxicity tests of silver nitrate (AgNO_3), silver chloride (AgCl_n), and silver thiosulfate (Ag(S_2O_3)_n) with juvenile rainbow trout (Oncorhyncus mykiss) were performed by standard methods. Because of low solubility of AgCl(s), bioassays for AgCl_n were performed in two separate ways. In one test series, AgCl(s) was added to freshwater and in another, AgCl_n(aq) was generated by adding AgNO_3 to freshwater supplemented with 50 mM NaCl. Concentrations of Ag and metallothionein (MT) were analyzed in gills and livers of fish that survived the exposures. Although Ag added as AgNO_3 was found to be highly toxic to rainbow trout (168-h LC50 = 9.1 μg Ag L~(-1)), the toxicities of the other Ag salts were low. The 168-h LC50 for Ag(S_2O_3)_n was 137,000 μg Ag L~(-1) and no mortality was observed in AgCl_n (100,000 μg Ag L~(-1)). Exposure to AgNO_3, Ag(S_2O_3)_n, or AgCl_n caused accumulation of Ag and induction of MT. Highest Ag levels were found in livers of trout exposed to 164,000 μg Ag L~(-1) as Ag(S_2O_3)_n. In these fish, the hepatic Ag concentration was increased 335 times from the control value. The MT levels in gills and liver increased with the water Ag concentration and the highest level of MT was found in liver of fish exposed to Ag(S_2O_3)_n.
机译：采用标准方法对硝酸银（AgNO_3），氯化银（AgCl_n）和硫代硫酸银（Ag（S_2O_3）_n）与幼年虹鳟（Oncorhyncus mykiss）进行了静态更新168小时毒性测试。由于AgCl的溶解度低，因此以两种不同的方式进行了AgCl_n的生物测定。在一个测试系列中，将AgCl添加到淡水中，在另一个测试系列中，通过将AgNO_3添加到补充有50 mM NaCl的淡水中来生成AgCl_n（aq）。在暴露后幸存的鱼的g和肝脏中分析了Ag和金属硫蛋白（MT）的浓度。尽管发现以AgNO_3形式添加的Ag对虹鳟鱼具有高毒性（168-h LC50 = 9.1μgAg L〜（-1）），但其他Ag盐的毒性却很低。 Ag（S_2O_3）_n的168h LC50为137,000μgAg L〜（-1），在AgCl_n（100,000μgAg L〜（-1））中未观察到死亡率。暴露于AgNO_3，Ag（S_2O_3）_n或AgCl_n会引起Ag的积累和MT的诱导。暴露于164,000μgAg L〜（-1）的鳟鱼肝脏中Ag含量最高，为Ag（S_2O_3）_n。在这些鱼中，肝中银的浓度比对照值增加了335倍。 the和肝脏中MT的含量随水中Ag浓度的增加而增加，而暴露于Ag（S_2O_3）_n的鱼肝中MT的含量最高。

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