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首页> 外文期刊>Environmental toxicology and chemistry >PHYTOTOXICITY, UPTAKE AND METABOLISM OF 1,4-DICHLOROBENZENE BY PLANT CELLS
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PHYTOTOXICITY, UPTAKE AND METABOLISM OF 1,4-DICHLOROBENZENE BY PLANT CELLS

机译:植物细胞对1,4-二氯苯的毒性,吸收和代谢。

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摘要

Phytotoxicity, uptake, and metabolism of 1,4-dichlorobenzene (1,4-DCB) by carrot (Daucus carota L.), soybean (Glycine max. L.), tomato (Lycopersicon esculentum Mill.), and red goosefoot (Chenopodiun rubrum L.) cell suspension cultures were studied. Sealed glass systems were utilized for the investigation because 1,4-DCB is volatile. The sealed systems affect the growth of plant cells, but do not provide different results when testing xenobiotic uptake and metabolism. 1,4-Dichlorobenzene (40 μg in 40 ml medium) was taken up by carrot (49%), soybean (50%), and red goosefoot (62%) cells. Only the soybean cell cultures provided evidence of the existence of metabolites of this compound, probably conjugates of chlorophenols. Conditions for phytotoxicity tests were modified because the growth of cell cultures was affected when sealed for longer than 2 d. 1,4-Dichlorobenzene is toxic to cell cultures of the three tested plant species (tomato, soybean, and carrot). Concentrations of 0.5 mM caused 50% growth inhibition in carrot and soybean cultures. The tomato cultures were more sensitive, with 0.05 mM causing 50% growth inhibition.
机译:胡萝卜(Daucus carota L.),大豆(Glycine max。L.),番茄(Lycopersicon esculentum Mill。)和红鹅掌(Chenopodiun)对1,4-二氯苯(1,4-DCB)的植物毒性,吸收和代谢研究了风疹细胞悬浮培养物。密封玻璃系统用于研究,因为1,4-DCB易挥发。密封的系统会影响植物细胞的生长,但在测试异源生物的摄取和代谢时不会提供不同的结果。 1,4-二氯苯(40毫升,在40毫升培养基中)被胡萝卜(49%),大豆(50%)和红鹅足(62%)细胞吸收。仅大豆细胞培养物提供了该化合物(可能是氯酚的结合物)代谢产物的存在的证据。修改了植物毒性试验的条件,因为密封超过2 d会影响细胞培养物的生长。 1,4-二氯苯对三种受试植物(番茄,大豆和胡萝卜)的细胞培养均具有毒性。 0.5 mM的浓度导致胡萝卜和大豆培养物中50%的生长抑制。番茄培养物更敏感,0.05 mM引起50%的生长抑制。

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