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首页> 外文期刊>Environmental toxicology and chemistry >MUTAGENIC POTENTIAL OF ENVIRONMENTAL SAMPLES BEFORE AND AFTER REMEDIATION OF A SOLVENT-CONTAMINATED SITE
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MUTAGENIC POTENTIAL OF ENVIRONMENTAL SAMPLES BEFORE AND AFTER REMEDIATION OF A SOLVENT-CONTAMINATED SITE

机译：溶剂污染场地修复前后的环境样品诱变潜力

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Soil, sediment, and paint sludge samples were collected during a 4-year period from an abandoned solvent recovery site prior to and after site remediation. Samples were sequentially extracted with methylene chloride and methanol and redis-solved in dimethyl sulfoxide for biological analysis. The mutagenic potential of the extracts was evaluated using the Salmonella/ microsome assay with and without metabolic activation. Noncontaminated background soil samples collected off-site induced specific mutagenic activities that ranged from below detection to 28 net revertants per milligram of residue. Although several sludge samples induced specific activities that were relatively low, the maximum mutagenic response induced by a sludge sample was 1,258 net revertants per milligram of residue. Compared to background soil, elevated levels of mutagenic activity were also detected in the extracts of several samples collected from a drainage area downgradient from the waste storage pad and in the extract of sediment samples from a downgradient storm-water retention pond. The weighted activity of the noncontami-nated background soil samples ranged from below detection to 15 revertants per gram of soil. The combined weighted activity of the sludge sample extracts ranged from below detection to 43,567 revertants per gram. The combined weighted activity of the extracts of samples collected after site remediation ranged from below detection to 1,273 revertants per gram. A visual inspection of the site following remediation suggested that all obvious signs of chemical contamination had been removed. However, the results from biological analysis indicate that soil collected following site remediation induced a weighted activity more than 30 times the maximum weighted activity observed in the noncontaminated background soil. These data suggest that additional site remediation is required if the genotoxic potential of soil is to be reduced to levels approximating those of noncontaminated background soil.
机译：在进行场地修复之前和之后的4年中，从废弃的溶剂回收场地收集了土壤，沉积物和油漆污泥样品。依次用二氯甲烷和甲醇萃取样品，然后重新溶解在二甲基亚砜中进行生物学分析。使用沙门氏菌/微粒体测定法在有或没有代谢活化的情况下评估提取物的诱变潜力。未污染的背景土壤样品收集了异地诱导的特定诱变活性，其活性从低于检测值到每毫克残留物28个净还原剂。尽管几种污泥样品诱导的比活相对较低，但由污泥样品诱导的最大诱变响应为每毫克残留物1,258净还原剂。与本底土壤相比，在从废物存储垫下流域下降的流域收集的一些样品的提取物中以及在下流雨水蓄积池的沉淀物样品的提取物中也检测到了诱变活性升高。非污染背景土壤样品的加权活性范围从低于检测值到每克土壤15个还原剂。污泥样品提取物的总加权活性范围从低于检测到每克43,567个还原剂。现场修复后收集的样品提取物的总加权活性范围从低于检测值到每克1,273个还原剂。修复后对现场进行的目视检查表明，已清除所有明显的化学污染迹象。但是，生物学分析的结果表明，在进行场地修复后收集的土壤引起的加权活动超过了在未污染的背景土壤中观察到的最大加权活动的30倍以上。这些数据表明，如果要将土壤的遗传毒性潜力降低到与未污染的背景土壤相似的水平，则需要进行其他的场地修复。

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