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首页> 外文期刊>Environmental toxicology and chemistry >A METHOD FOR PARTITIONING CADMIUM BIOACCUMULATED IN SMALL AQUATIC ORGANISMS
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A METHOD FOR PARTITIONING CADMIUM BIOACCUMULATED IN SMALL AQUATIC ORGANISMS

机译:一种对小型水生生物中累积的镉进行分区的方法

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A series of laboratory experiments was conducted to evaluate bioaccumulation and surface adsorption of aqueous cadmium (Cd) by sac-fry of the African tilapia Oreochromis niloticus. In the first experiment, the design consisted of two cadmium treatments: 15 μg Cd·L~(-1) in dilution water and a Cd-ethylenediaminetetraacetic acid (Cd-EDTA) complex at 15 μg·L~(-1), and a water-only control. There were five replicates per treatment and 40 fish per replicate. It was found that EDTA significantly reduced the bioaccumulation of cadmium by tilapia sac-fry by 34%. Based on these results, a second experiment was conducted to evaluate four procedures: a no-rinse control; rinsing in EDTA; rinsing in distilled water; and rinsing in 5% nitric acid, for removing surface-bound Cd from exposed sac-fry. In this experiment, 30 fish in each of five replicates were exposed to 15 μg Cd·L~(-1) for 72 h, processed through the rinse procedures, and analysed for total Cd. The EDTA rinse treatment significantly reduced (p < 0.05) Cd concentrations of the exposed fish relative to those receiving no rinse. It was concluded that the EDTA rinse technique may be useful in studies evaluating the partitioning of surface-bound and accumulated cadmium in small aquatic organisms.
机译:进行了一系列实验室实验,以评估非洲罗非鱼Oreochromis niloticus的囊泡对含水镉(Cd)的生物富集和表面吸附。在第一个实验中,设计包括两种镉处理:稀释水中15μgCd·L〜(-1)和15μg·L〜(-1)的Cd-乙二胺四乙酸(Cd-EDTA)络合物,以及一个纯水控件。每种处理有5个重复,每个重复有40条鱼。发现EDTA显着降低了罗非鱼囊对镉的生物富集34%。根据这些结果,进行了第二项实验,以评估四种程序:无冲洗控件;在EDTA中冲洗;用蒸馏水冲洗;并用5%的硝酸冲洗,以从暴露的网眼中除去表面结合的Cd。在该实验中,将五个重复样品中的每一个中的30条鱼暴露于15μgCd·L〜(-1)72小时,通过冲洗程序进行处理,并分析总Cd。相对于不进行漂洗的鱼类,EDTA漂洗处理显着降低了暴露鱼类的Cd浓度(p <0.05)。结论是,EDTA冲洗技术可用于评估小型水生生物中表面结合和积累的镉的分配的研究。

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