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首页> 外文期刊>Environmental toxicology and chemistry >Influence of Temperature and Nickel on Algal Biofilm Fatty Acid Composition
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Influence of Temperature and Nickel on Algal Biofilm Fatty Acid Composition

机译：温度和镍对藻类生物膜脂肪酸组成的影响

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Freshwater biofilms play an important role in aquatic ecosystems and are widely used to evaluate environmental conditions. Little is known about the effects of temperature and metals on biofilm fatty acid composition. In the present study, we exposed a natural biofilm cultured in mesocosms to a gradient of nickel (Ni) concentrations at 15 and 21 degrees C for 28 d. Metal bioaccumulation, algal taxonomic composition, and biofilm fatty acid profiles were determined. At both temperatures, bioaccumulated Ni increased with Ni exposure concentration and reached the highest values at 25 mu M Ni, followed by a decrease at 55 and 105 mu M Ni. In control biofilms, palmitic acid (16:0), palmitoleic acid (16:1n7), oleic acid (18:1n9), linoleic acid (18:2n6), and linolenic acid (18:3n3) were the dominant fatty acids at 15 and 21 degrees C. This composition suggests a dominance of cyanobacteria and green algae, which was subsequently confirmed by microscopic observations. The increase in temperature resulted in a decrease in the ratio of unsaturated to saturated fatty acids, which is considered to be an adaptive response to temperature variation. Polyunsaturated fatty acids (PUFAs) tended to decrease along the Ni gradient, as opposed to saturated fatty acids which increased with Ni concentrations. Temperature and Ni affected differently the estimated desaturase and elongase activities (product/precursor ratios). The increase in PUFAs at 15 degrees C was concomitant to an increase in Delta 9-desaturase (D9D). The estimated activities of D9D, Delta 12-desaturase, and Delta 15-desaturase decreased along the Ni gradient and reflected a decline in PUFAs. The elevated estimated elongase activity reflected the observed increase in saturated fatty acids at the highest Ni exposure concentration (105 mu M). Our results suggest that fatty acids could be used as an endpoint to evaluate environmental perturbations.Environ Toxicol Chem2020;00:1-12. (c) 2020 SETAC
机译：淡水生物膜在水生生态系统中发挥着重要作用，广泛用于评估环境条件。关于温度和金属对生物膜脂肪酸组成的影响很少。在本研究中，我们将在Mesocosms中培养的天然生物膜暴露于15至21℃的镍（Ni）浓度的梯度，28d。确定金属生物累积，藻类分类组合物和生物膜脂肪酸型材。在两个温度下，生物累计的Ni随NI曝光浓度而增加，并在25μmni时达到最高值，然后在55和105μmni下降。在对照生物膜中，棕榈酸（16：0），棕榈酸（16：1N7），油酸（18：1N9），亚油酸（18：2N6）和亚麻酸（18：3N3）是主要的脂肪酸该组合物建议该组合物提出了紫杉曲细菌和绿藻的优势，随后通过微观观察证实。温度的增加导致不饱和与饱和脂肪酸的比例的减少，这被认为是对温度变化的自适应响应。沿着Ni梯度倾向于降低的多不饱和脂肪酸（PUFA）与饱和脂肪酸相反，与Ni浓度增加。温度和Ni影响了估计的去饱和酶和延长酶活性（产品/前体比）。 15摄氏度的PUFA的增加伴随着Delta 9-去饱和酶（D9D）的增加。 D9D，Delta 12-去饱和酶和Delta 15-去饱和酶的估计活性沿着Ni梯度降低，并反映了Pufas的下降。升高的估计的延长酶活性反映了在最高Ni暴露浓度（105μm）上观察到的饱和脂肪酸的增加。我们的研究结果表明，脂肪酸可用作评估环境扰动的终点。紫罗兰毒素Chem202020; 00：1-12。 （c）2020 Setac

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