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首页> 外文期刊>Environmental toxicology and chemistry >Ibuprofen: Fish Short‐Term Reproduction Assay with Zebrafish (Danio rerio) Based on an Extended OECD 229 Protocol
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Ibuprofen: Fish Short‐Term Reproduction Assay with Zebrafish (Danio rerio) Based on an Extended OECD 229 Protocol

机译：布洛芬：基于扩展经合组织229协议，用斑马鱼（Danio Rerio）鱼短期再生测定

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A study was conducted to understand the potential for ibuprofen to impact the hypothalamus-pituitary-gonadal endocrine axis resulting in disruption of fish reproduction. The Good Laboratory Practice study was conducted according to the Organisation for Economic Co-operation and Development 229 Protocol, Fish Short-Term Reproduction Assay, and extended an additional 4 d to evaluate hatching success in the F1 generation. Test organisms were exposed to nominal test concentrations of 0.5, 2.4, 11.5, 55.3, and 265.4 mu g ibuprofen/L and a negative control (dilution water). To strengthen the statistical power of the study, twice the number of replicates were used in the negative control versus individual treatment levels. A 21-d pre-exposure to identify groups of actively spawning fish was immediately followed by a 36-d exposure. Results for apical endpoints of survival, growth, and reproduction (fecundity and fertility), as well as the biomarker vitellogenin in the F0 generation and time to hatch and hatching success in the F1 generation are presented. Based on mean measured exposure concentrations and effects on fecundity in the F0 generation and hatching success in the F1 generation, overall no-observed-effect concentration and lowest-observed-effect concentration for the present study were 55.2 and 265.9 mu g ibuprofen/L, respectively. Results from the present study indicate a lack of endocrine-mediated reproductive effects in zebrafish at environmentally relevant concentrations of ibuprofen.Environ Toxicol Chem2020;00:1-12. (c) 2020 SETAC
机译：进行了一项研究以了解布洛芬的潜力，以影响下丘脑 - 垂体 - 性腺内分泌轴，导致鱼繁殖中断。良好的实验室实践研究根据经济合作和发展组织进行229议定书，鱼短期繁殖试验，并扩展了4天，以评估F1代的孵化成功。将测试生物暴露于0.5,2.4,11.5,55.3和265.4μg的标称试验浓度为265.4μg，255.4μg，阴性对照（稀释水）。为了加强研究的统计能力，在阴性对照与个体治疗水平相比，使用两倍的重复次数。鉴定主动产卵鱼组的21-d预接触后立即接受36-D暴露。提出了生存，生长和繁殖（繁殖力和生育率）的顶点终点，以及F0代和孵化和孵化成功的生物标志物vitellogen in中的生物标志物vitellogen。基于平均测量的曝光浓度和对F0生成的孵化率的影响和对F1的成功的繁殖力，本研究的总体无观察到的效果浓度和最低观察到的效应浓度为55.2和265.9 mu g ubuprofen / l，分别。本研究结果表明，在环境相关浓度的布洛芬毒素中缺乏内分泌介导的斑马鱼生殖作用。00：1-12。 （c）2020 Setac

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