...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Environmental toxicology and chemistry >The Fabrication and the Use of Immobilized Cells as Test Organisms in a Ferricyanide-Based Toxicity Biosensor
【24h】

The Fabrication and the Use of Immobilized Cells as Test Organisms in a Ferricyanide-Based Toxicity Biosensor

机译：固定化细胞的制备及其在基于铁氰化物的毒性生物传感器中作为测试生物的用途

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Cell immobilization is an effective method to prolong the lifetime of a microorganism and has proven feasibility in some other biosensors. Thus, we studied the use of Escherichia coli immobilized by agar, gelatin, an agar/gelatin mixture, chitosan, and polyvinyl alcohol (PVA) to screen toxicity electrochemically. The E. coli immobilized by PVA gel showed the highest apparent bioactivity and the longest storage time in pH 7.0 phosphate-buffered saline solution. Furthermore, the E. coli immobilized by different gels was applied in the toxicity determination via a reported ferricyanide-mediated electrochemical method, where 3,5-dichlorophenol (DCP) was used as a model toxin. The E. coli immobilized by PVA showed the highest sensitivity to DCP, and the corresponding value of 50% inhibition concentration was 9.62 mgL(-1). Inhibition concentrations were in the range of 6.32 to 13.75% when the E. coli immobilized by PVA was challenged by wastewater, which were comparable with values obtained with the standard luminescent bacteria method (effective inhibition were in the range 7.96-25.42% for the same samples). Given the apparent bioactivity, storage ability, and sensitivity to toxin, PVA was the best polymer to confine cells among the polymers used in the present study. (C) 2017 SETAC
机译：细胞固定化是延长微生物寿命的有效方法，并且在某些其他生物传感器中已证明是可行的。因此，我们研究了琼脂，明胶，琼脂/明胶混合物，壳聚糖和聚乙烯醇（PVA）固定化的大肠杆菌在电化学上筛选毒性的用途。用PVA凝胶固定的大肠杆菌在pH 7.0磷酸盐缓冲盐溶液中表现出最高的表观生物活性和最长的存储时间。此外，通过报道的铁氰化物介导的电化学方法将通过不同凝胶固定化的大肠杆菌用于毒性测定，其中将3,5-二氯苯酚（DCP）用作模型毒素。 PVA固定化的大肠杆菌对DCP的敏感性最高，50％抑制浓度的相应值为9.62 mgL（-1）。当PVA固定化的大肠杆菌受到废水攻击时，抑制浓度在6.32％至13.75％的范围内，这与标准发光细菌方法获得的值相当（相同的有效抑制范围为7.96-25.42％样本）。鉴于其明显的生物活性，储存能力和对毒素的敏感性，在本研究中使用的聚合物中，PVA是限制细胞的最佳聚合物。 （C）2017年SETAC

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号