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首页> 外文期刊>Environmental toxicology and chemistry >Binding of Trace Elements (Ag, Cd, Co, Cu, Ni, and Tl) to Cytosolic Biomolecules in Livers of Juvenile Yellow Perch (Perca flavescens) Collected From Lakes Representing Metal Contamination Gradients
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Binding of Trace Elements (Ag, Cd, Co, Cu, Ni, and Tl) to Cytosolic Biomolecules in Livers of Juvenile Yellow Perch (Perca flavescens) Collected From Lakes Representing Metal Contamination Gradients

机译：从代表金属污染梯度的湖泊中收集的幼黄色鲈鱼（Perca flavescens）肝脏中的痕量元素（Ag，Cd，Co，Cu，Ni和Tl）与胞质生物分子的结合

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摘要

Biomolecules involved in handling cytosolic metals in the liver of the yellow perch (Perca flavescens) were characterized in juvenile fish collected from 4 lakes constituting metal contamination gradients. Using size-exclusion liquid chromatography coupled to an inductively coupled mass spectrometer, we determined metal distributions among ligands of different molecular weights in the cytosol, before and after a heat denaturation step designed to isolate metallothionein-like peptides and proteins. Silver, Cd, and Cu found in the heat-stable protein supernatants were indeed largely present as metallothionein-like peptide complexes; but Co, Ni, and Tl, also present in the heat-stable protein supernatants, did not coelute with metallothionein-like peptides and proteins. This difference in metal partitioning is consistent with the known preference of "soft" metals such as Ag, Cd, and Cu(I) for thiolated ligands and the contrasting tendency of Co and Ni to bind to ligands with oxygen and nitrogen as donor atoms. Metal handling in the whole cytosol also reflected these differences in metal-binding behavior. For Cd and Cu, the importance of the molecular weight pool that includes metallothionein-like peptides and proteins increased relative to the other pools as the total cytosolic metal concentration ([M](cytosol)) increased, consistent with a concentration-dependent detoxification response. In contrast, for Ni and Tl the increase in [M](cytosol) was accompanied by a marked increase in the high-molecular weight (670-33 kDa) pool, suggesting that hepatic Ni and Tl are not effectively detoxified. Overall, the results suggest that metal detoxification is less effective for Ni, Tl, and Co than for Ag, Cd, and Cu. (C) 2017 SETAC
机译：在从四个构成金属污染梯度的四个湖泊中收集的幼鱼中，对在黄色鲈鱼（Perca flavescens）肝脏中处理胞质金属的生物分子进行了表征。使用尺寸排阻液相色谱与电感耦合质谱仪联用，我们在设计用于分离金属硫蛋白样肽和蛋白质的热变性步骤之前和之后，确定了胞质溶胶中不同分子量配体之间的金属分布。在热稳定的蛋白质上清液中发现的银，镉和铜的确以金属硫蛋白样肽复合物的形式大量存在。但是在热稳定的蛋白质上清液中也存在Co，Ni和T1，它们不会与类金属硫蛋白的肽和蛋白质共洗脱。金属分配的这种差异与已知的“软”金属（例如Ag，Cd和Cu（I））对硫醇化的配体的偏爱以及Co和Ni与氧和氮作为供体原子的配体结合的对比趋势相一致。整个细胞质中的金属处理也反映了金属结合行为的这些差异。对于Cd和Cu，随着总胞溶金属浓度（[M]（cytosol））的增加，包含金属硫蛋白样肽和蛋白质的分子量池的重要性相对于其他池增加，这与浓度依赖性解毒反应一致。相反，对于Ni和T1，[M]（胞质溶胶）的增加伴随着高分子量（670-33 kDa）库的显着增加，这表明肝Ni和T1没有被有效地解毒。总体而言，结果表明，金属解毒对Ni，Tl和Co的效果不如对Ag，Cd和Cu的效果。 （C）2017年SETAC

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  • 来源
    《Environmental toxicology and chemistry》 |2018年第2期|576-586|共11页
  • 作者

    Caron Antoine; Rosabal Maikel; Drevet Ophelie; Couture Patrice; Campbell Peter G. C.;

  • 作者单位

    Inst Natl Rech Sci, Ctr Eau Terre Environm INRS ETE, Quebec City, PQ, Canada|Minist Dev Durable Environm & Lutte Changements C, Quebec City, PQ, Canada;

    Inst Natl Rech Sci, Ctr Eau Terre Environm INRS ETE, Quebec City, PQ, Canada|Univ Quebec Montreal, Dept Sci Biol, Montreal, PQ, Canada;

    Inst Natl Rech Sci, Ctr Eau Terre Environm INRS ETE, Quebec City, PQ, Canada|Com ZIP Seigneuries, St Sulpice, PQ, Canada;

    Inst Natl Rech Sci, Ctr Eau Terre Environm INRS ETE, Quebec City, PQ, Canada;

    Inst Natl Rech Sci, Ctr Eau Terre Environm INRS ETE, Quebec City, PQ, Canada;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Metal; Native fish; Liver; Detoxification; Lake;

    机译：金属;原生鱼;肝脏;排毒;湖泊;

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