Linking in vitro estrogenicity to adverse effects in the inland silverside (Menidia beryllina)

Mehinto Alvine C.; Kroll Kevin J.; Jayasinghe B. Sumith; Lavelle Candice M.; VanDervort Darcy; Adeyemo Olanike K.; Bay Steven M.; Maruya Keith A.; Denslow Nancy D.

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Linking in vitro estrogenicity to adverse effects in the inland silverside (Menidia beryllina)

机译：将体外雌激素作用与内陆银苷（Menidia beryllina）的不良影响联系起来

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High-throughput cell assays that detect and integrate the response of multiple chemicals acting via a common mode of action have the potential to enhance current environmental monitoring practices. Establishing the linkage between in vitro and in vivo responses is key to demonstrating that in vitro cell assays can be predictive of ecologically relevant outcomes. The present study investigated the potency of 17-estradiol (E2), estrone (E1), nonylphenol (NP), and treated wastewater effluent using the readily available GeneBLAzer((R)) estrogen receptor transactivation assay and 2 life stages of the inland silverside (Menidia beryllina). In vitro estrogenic potencies were ranked as follows: E2>E1NP. All 3 model estrogens induced vitellogenin and choriogenin expression in a dose-dependent manner in larvae and juveniles. However, apical effects were only found for E2 and E1 exposures of juveniles, which resulted in female-skewed sex ratios. Wastewater effluent samples exhibiting low in vitro estrogenicity (below the 10% effective concentration [EC10]), did not cause significant changes in M. beryllina. Significant induction of estrogen-responsive genes was observed at concentrations 6 to 26 times higher than in vitro responses. Gonadal feminization occurred at concentrations at least 19 to 26 times higher than the in vitro responses. These findings indicated that in vitro cell assays were more sensitive than the fish assays, making it possible to develop in vitro effect thresholds protective of aquatic organisms. Environ Toxicol Chem 2018;37:884-892. (c) 2017 SETAC

机译：检测并整合多种通过共同作用方式起作用的化学物质的反应的高通量细胞测定法有可能增强当前的环境监测实践。建立体外和体内反应之间的联系是证明体外细胞测定可以预测生态相关结果的关键。本研究使用现成的GeneBLAzer（R）雌激素受体反式激活测定法和内陆银杏苷的2个生命阶段，研究了17-雌二醇（E2），雌酮（E1），壬基酚（NP）和处理过的废水的效力。（Menidia beryllina）。体外雌激素效能排名如下：E2> E1 NP。所有3种模型雌激素在幼虫和幼虫中均以剂量依赖性方式诱导卵黄蛋白原和绒毛膜均蛋白的表达。但是，仅对未成年人的E2和E1暴露发现了根尖作用，这导致女性偏向性别比。废水中的样本显示出较低的雌激素性（低于10％有效浓度[EC10]），并没有引起Be.llina的明显变化。在比体外应答高6至26倍的浓度下观察到雌激素应答基因的显着诱导。性腺女性化的浓度至少比体外反应高19至26倍。这些发现表明，体外细胞试验比鱼类试验更为灵敏，从而有可能制定保护水生生物的体外效应阈值。 Environ Toxicol Chem 2018; 37：884-892。（c）2017年SETAC

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《Environmental toxicology and chemistry》 |2018年第3期|884-892|共9页
作者
Mehinto Alvine C.; Kroll Kevin J.; Jayasinghe B. Sumith; Lavelle Candice M.; VanDervort Darcy; Adeyemo Olanike K.; Bay Steven M.; Maruya Keith A.; Denslow Nancy D.;
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作者单位

Southern Calif Coastal Water Res Project Author S, Costa Mesa, CA 92626 USA;

Univ Florida, Dept Physiol Sci, Gainesville, FL 32610 USA|Univ Florida, Ctr Environm & Human Toxicol, Gainesville, FL 32611 USA;

Univ Florida, Dept Physiol Sci, Gainesville, FL 32610 USA|Univ Florida, Ctr Environm & Human Toxicol, Gainesville, FL 32611 USA;

Univ Florida, Dept Physiol Sci, Gainesville, FL 32610 USA|Univ Florida, Ctr Environm & Human Toxicol, Gainesville, FL 32611 USA;

Southern Calif Coastal Water Res Project Author S, Costa Mesa, CA 92626 USA;

Univ Florida, Dept Physiol Sci, Gainesville, FL 32610 USA|Univ Florida, Ctr Environm & Human Toxicol, Gainesville, FL 32611 USA;

Southern Calif Coastal Water Res Project Author S, Costa Mesa, CA 92626 USA;

Southern Calif Coastal Water Res Project Author S, Costa Mesa, CA 92626 USA;

Univ Florida, Dept Physiol Sci, Gainesville, FL 32610 USA|Univ Florida, Ctr Environm & Human Toxicol, Gainesville, FL 32611 USA;

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收录信息美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类
关键词
Estrogenic compounds; Adverse outcome pathway; Cell bioassays; Gene biomarkers; Gonadal feminization;

机译：雌激素化合物;不良结果途径;细胞生物测定;基因生物标志物;性腺女性化;

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1. Morphological and molecular variation among populations of tidewater (Menidia peninsulae) and inland (M-beryllina) silversides: insight into drivers of adaptation and speciation of silverside fishes [J] . Olsen Zachary, Anderson Joel, McDonald Dusty Environmental Biology of Fishes . 2016,第11期

机译：潮水（Menidia peninsulae）和内陆（M-beryllina）银杏种群之间的形态和分子变异：洞察银杏鱼类适应和物种形成的驱动因素
2. Trophic transfer, bioaccumulation and transcriptomic effects of permethrin in inland silversides, Menidia beryllina. under future climate scenarios [J] . Derby Andrew P., Fuller Neil W., Hartz Kara E. Huff, Environmental Pollution . 2021,第Apra期

机译：玻璃氟胺在内陆银霉素中的营养转移，生物累积和转录组效应，MENIDIA BERYLLINA。在未来的气候情景下
3. Effects of fractions from biodegraded Alaska North Slope crude oil on embryonic inland silversides, Menidia beryllina. [J] . Middaugh DP, ol.com, Chapman PJ, Archives of Environmental Contamination and Toxicology . 2002,第2期

机译：生物降解的阿拉斯加北坡原油馏分对内陆银杏胚芽，门尼迪亚（Menidia beryllina）的影响。
4. EFFECTS OF THYMOQUINONE, LYCOPENE, AND SELENOMETHIONE IN THE PRESENCE OF ESTROGEN ON THE VIABILITY OF SiHA CELLS in vitro [C] . Joyce Brewer, Hamed Benghuzzi, Michelle Tucci Annual Rocky Mountain Bioengineering Symposium . 2006

机译：紫醌，番茄红素，硒醌的影响在雌激素存在对雌激素的存在下体外生存性
5. The Impact of Temperature and Salinity on Bioconcentration of Permethrin in Hyalella azteca and Subsequent Bioaccumulation in Inland Silversides (Menidia beryllina) [D] . ?Derby, Andrew P. 2020

机译：温度和盐度对喀麦丽妥菌林甲虫霉素的生物浓缩的影响及随后在内陆银尔德（Menidia Beryllina）中的生物累计
6. Effects of Beauveria bassiana on embryos of the inland silverside fish (Menidia beryllina). [O] . F J Genthner, D P Middaugh 1992

机译：球孢白僵菌对内陆银边鱼（Menidia beryllina）胚胎的影响。
7. Effects of Beauveria bassiana on embryos of the inland silverside fish (Menidia beryllina). [O] . F J Genthner, D P Middaugh 1992

机译：Beauveria Bassiana对内陆硅藻（MENIDIA BERYLLINA）胚胎的影响。
8. Methods for Spawning, Culturing and Conducting Toxicity-Tests with Early Life Stages of Four Atherinid Fishes: The Inland Silverside, 'Menidia beryllina', Atlantic Silverside, 'M. menidia', Tidewater Silverside, 'M. peninsulae' and California Grunion, 'Leuresthes tenuis' [R] . Middaugh, D. P. , Hemmer, M. J. , Goodman, L. K. 1987

机译：产卵，培养和进行毒性试验的方法 - 四种atherinid鱼类的早期生命阶段：内陆银汉，'menidia beryllina'，atlantic silverside，'m。 menidia'，Tidewater silverside，'m。 peninsulae'和California Grunion，'Leuresthes tenuis'

Linking in vitro estrogenicity to adverse effects in the inland silverside (Menidia beryllina)

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