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首页> 外文期刊>Estuarine Coastal and Shelf Science >Effects Of Preservatives On Stable Isotope Analyses Of Four Marine Species
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Effects Of Preservatives On Stable Isotope Analyses Of Four Marine Species

机译：防腐剂对四种海洋物种稳定同位素分析的影响

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摘要

The aim of the present study is to quantify the effect of formalin-ethanol preservation on the carbon and nitrogen stable isotope signatures of four taxonomical groups of marine species (Himanthalia elongata. Ammonia sulcata, Mytilus galloprovincialis and Patella vulgata). To examine temporal changes in the effects of preservation and to determine if preservation induced predictable shifts in δ~(13)C and δ~(15)N signatures, repeated analyses were carried out after 6,12 and 24 months of preservation. Data from our study showed highly variable effects of the formalin-ethanol preservation on carbon and nitrogen isotope signatures between species. The use of a general correction factor was not possible, or else it should be species-specific. Differences in nitrogen isotopic values between preserved and unpreserved samples were minor compared to the assumed enrichment between trophic levels. The combined use of data from preserved and unpreserved samples could lead to biases in the estimation of the trophic level of organisms. Changes that preservatives caused in carbon values were variable between species and not always small enough to be ignored. So the use of data from preserved samples could change the interpretation of the mixing models used to determine the importance of multiple sources of carbon. In order to elucidate the effects that preservatives have in other species, further studies will be necessary.
机译：本研究的目的是定量福尔马林-乙醇保存对四种海洋生物分类群（Himanthalia elongata。sulcata sulcata，Mytilus galloprovincialis和Vulgata）碳和氮稳定同位素特征的影响。为了检查保存效果的时间变化并确定保存是否导致δ〜（13）C和δ〜（15）N签名发生可预测的变化，在保存6,12和24个月后进行了重复分析。我们研究的数据显示，福尔马林-乙醇保存对物种之间碳和氮同位素特征的影响变化很大。不能使用一般的校正因子，否则应该是特定于物种的。与假定营养水平之间的富集相比，保存和未保存样品之间的氮同位素值差异很小。来自保存和未保存样品的数据的组合使用可能导致估计生物的营养水平。防腐剂在碳含量上引起的变化在不同物种之间是可变的，并不总是小到可以忽略的程度。因此，使用保存样本中的数据可以改变混合模型的解释，该模型用于确定多种碳源的重要性。为了阐明防腐剂在其他物种中的作用，将需要进一步的研究。

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