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首页> 外文期刊>Estuarine Coastal and Shelf Science >Bacterial communities in Great Barrier Reef calcareous sediments: Contrasting 16S rDNA libraries from nearshore and outer shelf reefs
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Bacterial communities in Great Barrier Reef calcareous sediments: Contrasting 16S rDNA libraries from nearshore and outer shelf reefs

机译：大堡礁钙质沉积物中的细菌群落：来自近岸和外层架礁的16S rDNA库对比

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Bacterial communities in eight 16S rDNA clone libraries from calcareous sediments were investigated to provide an assessment of the bacterial diversity on sediments of the Great Barrier Reef (GBR) and to investigate differences due to decreased water quality. Sample effort was spread across two locations on each of four coral reefs, with two reefs located nearshore and two reefs on the outer shelf to allow robust statistical comparison of nearshore reefs (subjected to enhanced runoff) and outer shelf reefs (pristine conditions). Out of 221 non-chimeric sequences, 189 (85.5%) were unique and only one sequence occurred in more than one library. Rarefaction analyses and coverage calculations indicated that only a small fraction of the diversity was sampled. Cluster analyses and comparison to published sequences indicated that sequences retrieved belonged to the α, γ and δ subdivision of the Proteobacteria (6.8, 29.4 and 13.6% of the total, respectively), Cytophaga-Favobacteria-Bacteroidetes (CFB) group (20.4%), Cyanobacteria (5.4%), Planctomycetaceae (7.7%), Verrucomicrobiaceae (6.8%), Acidobacteriaceae (2.7%). Analysis of Similarity (ANOSIM, based on grouping all retrieved sequences into 9 phylogenetic groups) indicated that subtle differences do exist in the community composition between nearshore and outer shelf reefs. Similarity percentage analysis (SIMPER) indicated that Acidobacteriaceae and Cyanobacteriaceae were the main contributors to the dissimilarity. A significant difference between bacteria on nearshore and outer shelf reefs also existed on the molecular level (F_(ST) = 0.008, p = 0.007 for all samples, 0.006, p = 0.022 when repeated sequences within libraries were removed). Thus, bacterial communities on carbonate sediments investigated were highly diverse and differences in community composition may provide important leads for the search for indicator species or communities for water quality differences.
机译：调查了来自钙质沉积物的八个16S rDNA克隆文库中的细菌群落，以评估大堡礁（GBR）沉积物上的细菌多样性，并调查由于水质下降而造成的差异。样本工作量分布在四个珊瑚礁中每个的两个位置上，其中两个礁石位于近岸，而在外层架上有两个礁石，从而可以对近岸礁石（受径流量增加影响）和外层礁石（原始条件）进行可靠的统计比较。在221个非嵌合序列中，有189个（85.5％）是唯一的，并且在一个以上的文库中仅出现了一个序列。反射分析和覆盖率计算表明，仅对多样性的一小部分进行了采样。聚类分析和与已发表序列的比较表明，检索到的序列分别属于变形杆菌的α，γ和δ细分（分别占总数的6.8、29.4和13.6％），细胞吞噬-嗜细菌-拟杆菌（CFB）组（占20.4％） ，蓝细菌（5.4％），扁平菌科（7.7％），疣状微生物菌科（6.8％），酸菌科（2.7％）。相似性分析（ANOSIM，基于将所有检索到的序列分为9个系统发生类群）表明，近岸礁和外层礁之间的群落组成确实存在细微差异。相似度百分比分析（SIMPER）表明，嗜酸菌科和蓝藻科是造成这种差异的主要原因。在分子水平上，近岸礁和外层礁的细菌之间也存在显着差异（F_（ST）= 0.008，所有样品的p = 0.007，去除文库中的重复序列时为0.006，p = 0.022）。因此，调查的碳酸盐沉积物上的细菌群落高度多样，群落组成的差异可能为寻找指示物种或水质差异的群落提供重要线索。

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