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首页> 外文期刊>Euphytica >Genetic studies in Centrosema pubescens benth, a tropical forage legume: the mating system, genetic variability and genetic relationships between Centrosema species
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Genetic studies in Centrosema pubescens benth, a tropical forage legume: the mating system, genetic variability and genetic relationships between Centrosema species

机译：热带食草豆类中心线虫的遗传研究：中心线虫种之间的交配系统，遗传变异和遗传关系

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摘要

In this study, we used microsatellite loci to estimate the outcrossing rate of Centrosema pubescens in open-pollinated populations of 10 progenies that each contained 20 genotypes. The multilocus outcrossing rate was 27%, which suggested a mixed mating system with a predominance of autogamy. The single locus outcrossing rate was 13%. The difference was 0.040, which indicated that only 4% of outcrossing occurs in related individuals. A paternity correlation of 14% suggests that there is a low probability of finding full sibs in the progeny. Cross-amplification of the 26 microsatellite loci available for C. pubescens was evaluated across 11 different Centrosema species. Nineteen of the 26 tested microsatellites were successfully transferable across the Centrosema species. The polymorphism information content and discriminating power evaluated had averages of 0.64 and 0.77, respectively. A total of three clusters were assembled to demonstrate the genetic relationships between Centrosema species. The transferable microsatellite loci should be useful for exploiting the genetic resources of the Centrosema species and determining the outcrossing rate, which are essential for proposing effective approaches for conservation and for establishing strategies for the selection and improvement of Centrosema spp.
机译：在这项研究中，我们使用微卫星基因座来评估在10个子代的开放授粉种群中毛状中心的异交率，每个子代包含20个基因型。多基因座异交率是27％，这表明混合配种系统以自配性为主。单基因座异源率是13％。差异为0.040，这表明只有4％的异型杂交发生在相关个体中。 14％的亲子关系表明在后代中发现完整同胞的可能性很小。可在11种不同的Centrosema物种中评估pubescens的26个微卫星基因座的交叉扩增。在测试的26个微卫星中，有19个可成功转移到Centrosema物种上。评估的多态信息含量和鉴别力的平均值分别为0.64和0.77。总共组装了三个簇，以证明中心型种之间的遗传关系。可转移的微卫星基因座应有助于开发中心线虫物种的遗传资源和确定异源率，这对于提出有效的保护方法以及建立中心线虫的选择和改良策略至关重要。

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  • 来源
    《Euphytica》 |2011年第2期|p.223-235|共13页
  • 作者

    A. C. B. Sousa; M. A. Carvalho; A. K. B. Ramos; T. Campos; D. A. Sforça; M. I. Zucchi; L. Jank; A. P. Souza;

  • 作者单位

    Brazilian Agricultural Research Corporation, EMBRAPA Cerrados, BR 020, Km 18, Planaltina, DF, CEP 73310-970, Brazil;

    Brazilian Agricultural Research Corporation, EMBRAPA Cerrados, BR 020, Km 18, Planaltina, DF, CEP 73310-970, Brazil;

    Agronomic Institute of Campinas, Pólo Apta Centro Sul, Rod. SP 127 Km 30, CP 28, Piracicaba, SP, CEP 13400-970, Brazil;

    Forage Breeding Department, Brazilian Agricultural Research Corporation, EMBRAPA Beef Cattle, CP 154, Campo Grande, MS, CEP 79002-970, Brazil;

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  • 正文语种 eng
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  • 关键词

    Tropical legume; Cross-amplification; Mating system; Autogamy;

    机译：热带豆科植物;交叉扩增;交配系统;配子;

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