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Identification and molecular mapping of a leaf rust resistance gene in spelt wheat landrace Altgold

机译:小麦小麦地方品种Altgold中抗叶锈基因的鉴定和分子定位

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摘要

Leaf rust, caused by Puccinia triticina, is an important disease for wheat production, both in China and worldwide. In laboratory studies spelt wheat (Triticum aestivum ssp. spelta) landrace Altgold was resistant to P. triticina races THT and PHT and genetic analysis indicated that it possessed a dominant leaf rust resistance gene, temporarily designated LrAlt. F6 recombinant inbred lines (RILs) derived from a cross with the susceptible common wheat cultivar Nongda 3338 were used to map LrAlt with SSR markers. The resistance gene was distal to SSR loci Xbarc212, Xwmc382, Xgwm636, and Xwmc407 on the short arm of chromosome 2A. The closest markers Xbarc212 and Xwmc382 which co-segregated were 1.8 cM away from LrAlt. The relationships of LrAlt and other wheat leaf rust resistance genes located on the short arm of chromosome 2A were discussed, suggesting that LrAlt might be a new leaf rust resistance gene.
机译:由小麦锈菌引起的叶锈病,在中国乃至全球都是小麦生产的重要疾病。在实验室研究中,拼写小麦(Triticum aestivum ssp。spelta)的地方品种Altgold对小麦P. triticina小种THT和PHT具有抗性,遗传分析表明它具有显性的叶锈病抗性基因,临时命名为LrAlt。利用与易感普通小麦品种农大3338杂交获得的F 6 重组近交系(RIL),对带有SSR标记的LrAlt作图。抗性基因位于2A染色体短臂上的SSR基因座Xbarc212,Xwmc382,Xgwm636和Xwmc407的远端。共分离的最接近的标记Xbarc212和Xwmc382与LrAlt相距1.8 cM。讨论了LrAlt基因与其他位于2A染色体短臂上的小麦叶片抗锈基因的关系,表明LrAlt可能是一个新的叶片抗锈基因。

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