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首页> 外文期刊>Euphytica >Resistance to papaya ringspot virus in summer squash Cucurbita pepo L. introgressed from an interspecific C. pepo × C. moschata cross
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Resistance to papaya ringspot virus in summer squash Cucurbita pepo L. introgressed from an interspecific C. pepo × C. moschata cross

机译：从种间C. pepo×C. moschata杂交渗入的西葫芦西葫芦对木瓜环斑病毒的抗性

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摘要

Early infections of the papaya ringspotvirus-watermelon strain (PRSV-W) in summersquash Cucurbita pepo may causemarketable yield losses of up to 100%. Because control of the viral disease viamanagement of vector insects is notefficient, incorporation of PRSV-W geneticresistance into C. pepo cultivarswould be highly desirable. Althoughresistant accessions have been described inC. ecuadorensis, C.foetidissima, C. maxima and C.moschata, resistant accessions of C.pepo are rare. This paper reports oninterspecific hybridization between C.pepo and a PRSV-W-resistant C.moschata squash accession, and on theearly steps in introgression of thisresistance into the former species. C. pepo cv. Asmara was crossed with twoPRSV-W resistant C. moschataaccessions. Immature F1 (C.pepo× C.moschata) embryos were excised20 days after pollination, and culturedasseptically in two different culturemedia, each under two different lightregimes. No adult plants could berecovered from immature embryos of thecross C. pepo cv. Asmara × C.moschata cv. Piramoita. From the crossC. pepo cv. Asmara × C.moschata cv. Duda, 4 seedlings wererecovered from 6 embryos incubated in agrowth chamber [25° to 28 °C,16 hours of light with 1500–2000 lux/ 8 hdark period] immediately after excision andinoculation in a culture medium [=Murashige and Skoog (MS) + 0.01 mg/L of IAA(indolacetic acid) + 0.1 mg/L of Kinetin],without an intervening dark period. F1 (C. pepo × C. moschata)plants were fertile, and upon selfingyielded viable F2 seeds. PRSV-Wresistance was assessed in the F2 (C. pepo × C. moschata) population,using C. pepo cv. Asmara and C.moschata cv. Duda, respectively, as thesusceptible and resistant (tolerant)checks. Plants were mechanically inoculatedwith a known PRSV-W isolate. The F2population showed a PRSV-W responseintermediate between those of the parentalaccessions, and estimates of the meandegree of dominance indicates incompletedominance in the direction ofsusceptibility. The mean broad-senseheritability estimate of PRSV-W resistancewas 0.48, a medium to high value,indicating the likelihood of a significantresponse to selection for resistance. Theintrogression of the resistance to PRSV-Wof C. moschata into C. peposeems to be technically viable.
机译：在西葫芦南瓜中早期感染木瓜环斑病毒西瓜菌株（PRSV-W）可能导致高达100％的可销售产量损失。由于通过媒介昆虫的控制来控制病毒性疾病效率不高，因此非常需要将PRSV-W遗传抗性纳入到C. pepo栽培种中。尽管在C中描述了抗性登录。 ecuadorensis，C.foetidissima，C.maxima和C.moschata很少有C.pepo的抗性种质。本文报道了C.pepo和抗PRSV-W的C.moschata南瓜种间的种间杂交，以及这种抗性渗入前种的早期步骤。 C. pepo简历阿斯马拉与两种抗PRSV-W的毛孢梭菌杂交。授粉后20天将未成熟的F1 （C.pepo×C.moschata）胚胎切除，并在两种不同的光环境下，在两种不同的培养基中无菌培养。从交配的C. pepo cv的未成熟胚中不能回收到成年植物。阿斯马拉×C.moschata cv。皮拉莫伊塔从十字架C.佩珀简历阿斯马拉×C.moschata cv。刚切除并接种在培养基中后，立即在生长室中[25°至28°C，在1500–2000 lux / 8黑暗的时间里光照16小时]从6个胚中回收了Duda的4株幼苗。[Murashige and Skoog（MS） + 0.01 mg / L的IAA（吲哚乙酸）+ 0.1 mg / L的Kinetin]，无中间黑暗期。 F1 （C。pepo×C. moschata）植物肥沃，自成活的F2 种子。使用C. pepo cv对F2 （C. pepo×C. moschata）人群进行PRSV耐药性评估。 Asmara和C.moschata简历。杜达分别作为易感和耐药（耐受）检查。用已知的PRSV-W分离物机械接种植物。 F2 人群在亲本种之间表现出PRSV-W应答中间性，对中位优势度的估计表明在易感性方向上不完全主导。 PRSV-W抗性的平均广义可遗传性估计值为0.48，是一个中到高值，表明对抗性选择产生重大响应的可能性。从技术上讲，将对毛圆线虫的PRSV-W的抗性渗入pe.emposeems是可行的。

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