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首页> 外文期刊>Euphytica >Improvement of somatic embryogenesis and plant regeneration from durum wheat (Triticum turgidum var. durum Desf.) scutellum and inflorescence cultures
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Improvement of somatic embryogenesis and plant regeneration from durum wheat (Triticum turgidum var. durum Desf.) scutellum and inflorescence cultures

机译：改善硬粒小麦（盾粒小麦）盾片和花序培养的体细胞胚发生和植物再生

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Scutellum and inflorescence explants of four genotypes of durum wheat(Triticum turgidum var. durum Desf.) were used to define culture conditions to obtain high frequencies of embryogenesis and plant regeneration in vitro. Under all conditions tested, scutellum cultures gave higher frequencies of embryogenesis and plant regeneration than inflorescence cultures. Two different auxins, 2,4-D(2,4-dichlorophenoxyacetic acid) and picloram(4-amino-3,5,6-trichloropicolinic acid), were compared for their effect on scutellum and inflorescence explant response in vitro. Picloram was found to significantly increase the frequency of plant regeneration from both explants. When cultures were grown on regeneration medium containing zeatin for two three-week passages, the frequency of plant regeneration increased by between 20–30% compared with cultures exposed to hormones for a single three-week passage. Finally, the addition of 1 mg/l 6-BAP (6-benzyl aminopurine) to the plantlet growth medium was found to enhance tiller production in regenerants. The optimized culture conditions were applicable to the four genotypes tested and frequencies of plant regeneration varied between 97% to 100% for scutellum cultures (2 mg/l picloram in induction medium) and between45% and 80% for inflorescence cultures (4 mg/l picloram in induction medium). The number of plants regenerated per explant was improved over previous procedures, with means of 34 plants per scutellum, and 16 plants per inflorescence explant.
机译：利用四种基因型硬粒小麦的盾片和花序外植体确定培养条件，以获得高频率的胚发生和植物再生。在所有测试条件下，盾片培养的花序发生和植株再生的频率均高于花序培养。比较了两种不同的生长素，即2,4-D（2,4-二氯苯氧基乙酸）和吡咯烷（4-氨基-3,5,6-三氯吡啶甲酸）对盾片和花序外植体响应的影响。发现Picloram显着增加了两种外植体的植物再生频率。当培养物在含有玉米蛋白的再生培养基上生长两次，历时三周时，与暴露于激素中的培养物经过三周时相比，植物再生的频率增加了20％至30％。最后，发现向小苗生长培养基中添加1 mg / l 6-BAP（6-苄基氨基嘌呤）可提高再生剂中的分till产量。优化的培养条件适用于测试的四种基因型，盾片培养（诱导培养基中2 mg / l吡咯仑）的植物再生频率在97％至100％之间，而花序培养（4 mg / l）的植物再生频率在45％至80％之间变化吡咯烷在诱导培养基中）。每个外植体再生的植物数量比以前的程序有所改善，平均每个盾片34个植物，每个花序外植体16个植物。

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