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首页> 外文期刊>European Journal of Plant Pathology >How can we exploit functional genomics approaches for understanding the nature of plant defences? Barley as a case study
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How can we exploit functional genomics approaches for understanding the nature of plant defences? Barley as a case study

机译：我们如何利用功能基因组学方法来了解植物防御的本质？大麦作为案例研究

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摘要

The concept ‘functional genomics’ refers to the methods used for the functional characterisation of genomes. The methods utilised provide new opportunities for studying the nature and role of defence mechanisms in plants. Unlike Arabidopsis, poplar and rice, the full genomic sequence of barley is not available. In this case, the analysis of barley gene expression data plays a pivotal role for obtaining insight into the functional characterisation of individual gene products. Many genes are activated transcriptionally following attack by pathogens and these often contribute to the defence mechanisms which underlie disease resistance. The use of large-scale complementary DNA library constructions and genome-wide transcript profiles of plants exposed to biotic stress provide the data required to drive hypotheses concerning the function of newly identified genes. In this paper, we illustrate how publicly available gene expression data has proved valid for studies of plant defence responses; enabling a cost-effective workflow starting from isolated gene transcripts to elucidation of biological function upon biotic stress.
机译：“功能基因组学”概念是指用于基因组功能表征的方法。利用的方法为研究植物防御机制的性质和作用提供了新的机会。与拟南芥，杨树和水稻不同，大麦没有完整的基因组序列。在这种情况下，大麦基因表达数据的分析对于深入了解单个基因产物的功能特性起着至关重要的作用。许多基因在病原体攻击后被转录激活，这些基因通常有助于构成抗病性的防御机制。暴露于生物胁迫下的植物的大规模互补DNA文库构建和全基因组转录本谱的使用提供了驱动有关新鉴定基因功能的假设所需的数据。在本文中，我们说明了公开可用的基因表达数据如何证明对植物防御反应的研究有效；从分离的基因转录本到阐明生物胁迫时的生物学功能，实现经济高效的工作流程。

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