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Activity-driven fluctuations in living cells

机译:活动驱动的活细胞波动

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Living cells operate far from equilibrium due to the permanent injection of energy provided by ATP supply. The dynamics of the intracellular components is driven by both thermal equilibrium fluctuations, and active stochastic forces generated by the molecular motors. To sort out genuine nonequilibrium fluctuations from purely thermal effects, we inject tracer particles in ATP depleted cells. By testing the fluctuation-dissipation theorem (FDT), we identify these cells as an equilibrium-like reference in which the tracers remain locally confined by the elastic cytoskeletal network that permeates the cytoplasm. In contrast, we highlight a violation of the FDTand a diffusion-like motion at long time scales in untreated and selective motor inhibited cells.
机译:由于ATP供应所提供的永久性能量注入,活细胞的工作远未达到平衡。细胞内组分的动力学受分子温度产生的热平衡波动和主动随机力的驱动。为了从纯粹的热效应中找出真正的非平衡波动,我们在ATP耗尽的细胞中注入示踪剂颗粒。通过测试波动耗散定理(FDT),我们将这些细胞鉴定为类似平衡的参考,其中示踪剂仍被渗透到细胞质中的弹性细胞骨架网络局限在局部。相比之下,我们强调了在未经治疗和选择性运动抑制的细胞中,在较长的时间尺度上违反了FDT和类似扩散的运动。

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