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首页> 外文期刊>Experimental Animals >Effects of Intestinal Microflora on Superoxide Dismutase Activity in the Mouse Cecum
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Effects of Intestinal Microflora on Superoxide Dismutase Activity in the Mouse Cecum

机译：肠道菌群对小鼠盲肠超氧化物歧化酶活性的影响

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摘要

In the antioxidant defense system, superoxide dismutase (SOD) catalyzes the breakdown of superoxide into hydrogen peroxide and oxygen. In the cecum, the influence of intestinal microflora on SOD activity is unknown. In this study, we used germ-free (GF) mice to examine the effect of intestinal microflora on SOD activity in the cecum, and SOD activity was compared between GF and conventional (CV) mice. The activity of CuZnSOD and MnSOD was determined using the SOD Assay Kit-WST. Expressions of CuZnSOD mRNA and protein were determined by real-time PCR and western blot analyses, respectively. The activities of CuZnSOD and MnSOD were significantly higher in the ceca of GF IQI and FVB/N strain mice than in CV mice (P<0.01-0.05). The gene expressions of CuZnSOD mRNA in the ceca of GF mice were significantly higher than those in CV mice (P<0.05), and CuZnSOD protein expression showed similar tendencies. Consistent with the abovementioned results, the total SOD activity in conventionalized mice decreased to the level of total SOD activity observed in the ceca of CV mice. Furthermore, no differences between GF and CV mice were observed in the SOD activities in the liver and thymus. Our results suggest that the antioxidant defense system in the mouse cecum is influenced by the intestinal microflora that downregulate SOD activity.
机译：在抗氧化剂防御系统中，超氧化物歧化酶（SOD）催化超氧化物分解为过氧化氢和氧气。在盲肠中，肠道菌群对SOD活性的影响尚不清楚。在这项研究中，我们使用无菌（GF）小鼠检查肠道菌群对盲肠SOD活性的影响，并比较了GF和常规（CV）小鼠的SOD活性。使用SOD测定试剂盒-WST测定了CuZnSOD和MnSOD的活性。分别通过实时PCR和western blot分析确定CuZnSOD mRNA和蛋白的表达。 GF IQI和FVB / N品系小鼠的盲肠中的CuZnSOD和MnSOD活性明显高于CV小鼠（P <0.01-0.05）。 GF小鼠盲肠中CuZnSOD mRNA的基因表达明显高于CV小鼠（P <0.05），并且CuZnSOD蛋白的表达趋势相似。与上述结果一致，常规小鼠中的总SOD活性降低到CV小鼠盲肠中观察到的总SOD活性水平。此外，在GF和CV小鼠之间，在肝脏和胸腺中的SOD活性未见差异。我们的结果表明，小鼠盲肠中的抗氧化防御系统受到下调SOD活性的肠道菌群的影响。

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  • 来源
    《Experimental Animals》 |2011年第2期|p.133-139|共7页
  • 作者

    Yuu DOBASHI; Yoshihiko MIYAKAWA; Ichiro YAMAMOTO; Hiromi AMAO;

  • 作者单位

    Laboratory of Experimental Animal Science, Nippon Veterinary and Animal Science University, 1-7-1 Kyonan-cho, Musashino, Tokyo 180-8602,Japan;

    rnInstitute of Laboratory Animals, Tokyo Women's Medical University, 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8666,Japan;

    rnLaboratory of Veterinary Biochemistry, Nippon Veterinary and Animal Science University, 1-7-1 Kyonan-cho,Musashino, Tokyo 180-8602, Japan;

    rnLaboratory of Experimental Animal Science, Nippon Veterinary and Animal Science University, 1-7-1 Kyonan-cho, Musashino, Tokyo 180-8602,Japan;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    cecum; germ-free; intestinal microflora; superoxide dismutase;

    机译：盲肠无菌肠道菌群超氧化物歧化酶;

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