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首页> 外文期刊>Experimental Animals >Transgenic Mouse Sperm that Have Green Acrosome and Red Mitochondria Allow Visualization of Sperm and Their Acrosome Reaction in Vivo
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Transgenic Mouse Sperm that Have Green Acrosome and Red Mitochondria Allow Visualization of Sperm and Their Acrosome Reaction in Vivo

机译:具有绿色顶体和红色线粒体的转基因小鼠精子可以观察到精子及其体内的顶体反应

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摘要

In the present paper, we introduce a transgenic mouse line whose sperm express green fluorescent protein (GFP) in their acrosome and red fluorescent protein (RFP) in their mitochondria [B6D2F1- Tg(CAG/su9-DsRed2, Acr3-EGFP)RBGS002Osb]. The dual fluorescent sperm showed normal fertilizing ability in both in vivo and in vitro fertilization and the sperm could be observed through uterine and oviductal walls when female reproductive tracts were dissected out and placed under excitation light. This characteristic could facilitate examination of sperm migration inside the female reproductive tract as well as facilitating in situ live imaging of the acrosome reaction, the details of which have remained elusive.
机译:在本文中,我们介绍了一种转基因小鼠品系,其精子在顶体中表达绿色荧光蛋白(GFP),在线粒体中表达红色荧光蛋白(RFP)[B6D2F1- Tg(CAG / su9-DsRed2,Acr3-EGFP)RBGS002Osb] 。双荧光精子在体内和体外受精中均显示出正常的受精能力,当解剖女性生殖道并将其置于激发光下时,可以通过子宫和输卵管壁观察到该精子。该特征可以促进检查精子在女性生殖道内的迁移,并促进顶体反应的原位实时成像,其细节仍难以捉摸。

著录项

  • 来源
    《Experimental Animals》 |2010年第1期|105-107|共3页
  • 作者单位

    Genome Information Research Center, Research Institute for Microbial Diseases, Osaka University,3-1 Yamadaoka, Suita, Osaka 565-0871, Japan;

    Genome Information Research Center, Research Institute for Microbial Diseases, Osaka University,3-1 Yamadaoka, Suita, Osaka 565-0871, Japan;

    Genome Information Research Center, Research Institute for Microbial Diseases, Osaka University,3-1 Yamadaoka, Suita, Osaka 565-0871, Japan;

    Laboratory of Molecular Genetics, Department of Medical Genetics, Osaka University Medical School, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan JCL Bioassay Corp., 17-18 Nakahata-cho, Nishiwaki, Hyogo 677-0032, Japan;

    Laboratory of Molecular Genetics, Department of Medical Genetics, Osaka University Medical School, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan;

    Genome Information Research Center, Research Institute for Microbial Diseases, Osaka University,3-1 Yamadaoka, Suita, Osaka 565-0871, Japan;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    live imaging; oviduct; sperm;

    机译:实时成像;输卵管;精子;

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