...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Experimental Animals >Cryopreservation for Broader Production of Transgenic Mice by DNA Injection into Zygotes
【24h】

Cryopreservation for Broader Production of Transgenic Mice by DNA Injection into Zygotes

机译：冷冻保存，通过向合子中注射DNA来广泛生产转基因小鼠

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Mutant mice are indispensable to biological and medical research, and transgenic mouse production by DNA injection into zygotes has been an important method for producing these mice which are used to examine the over-expression of genes and to analyze gene transcriptional regulatory sequences. Recently, cryopreservation of zygotes by a simple vitrification method has become popular, saving the labor of isolating zygotes following each injection. However, the DNA injection technique requires training in the use of special equipment, and the injection cannot be accomplished routinely in every laboratory. The exchange of live mice also risks the propagation of common murine pathogens and possible escape of the animals; therefore the transfer of mice in frozen zygotes or embryos is recommended. Here we propose injecting DNA into frozen and thawed zygotes, refreezing them before transportation to any destination, where they can be thawed and developed into pups. The rate of transgenic mouse production using this method does not decrease significantly, even if zygotes are frozen and thawed before and after their DNA injection, and will make transgenic studies more popular on a worldwide scale.
机译：突变小鼠是生物学和医学研究必不可少的，通过将DNA注入合子中来生产转基因小鼠已经成为生产这些小鼠的重要方法，这些小鼠用于检查基因的过表达和分析基因转录调控序列。近来，通过简单的玻璃化方法冷冻保存受精卵已变得流行，节省了每次注射后分离受精卵的工作。但是，DNA注射技术需要使用特殊设备的培训，并且注射不能在每个实验室中常规完成。交换活小鼠还冒着普通鼠类病原体繁殖和动物可能逃脱的危险；因此，建议在冷冻的受精卵或胚胎中转移小鼠。在这里，我们建议将DNA注入冷冻和解冻的合子中，在运输到任何可以解冻并发育成幼仔的目的地之前将其重新冷冻。即使将合子在注射DNA之前和之后冷冻并解冻，使用这种方法产生的转基因小鼠的速率也不会显着降低，这将使转基因研究在全球范围内更加普及。

著录项

  • 来源
    《Experimental Animals》 |2010年第2期|p.225-229|共5页
  • 作者

    Kazuki NAKAO; Kenichi INOUE;

  • 作者单位

    Laboratory for Animal Resources and Genetic Engineering, RIKEN Center for Developmental Biology, 2-2-3 Minatojima-Minamimachi, Chuo-ku, Kobe 650-0047 Division of Molecular Genetics, Department of Physiology and Cell Biology, Kobe University Graduate School of Medicine, 7-5-1 Kusunokichou, Chuo-ku, Kobe 650-0017, Japan;

    Laboratory for Animal Resources and Genetic Engineering, RIKEN Center for Developmental Biology, 2-2-3 Minatojima-Minamimachi, Chuo-ku, Kobe 650-0047;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    cryopreservation; DNA injection; mouse; transgenic; vitrification;

    机译：冷冻保存DNA注射;老鼠;转基因玻璃化;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号