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首页> 外文期刊>Experimental Animals >Xenopus tropicalis: An Ideal Experimental Animal in Amphibia
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Xenopus tropicalis: An Ideal Experimental Animal in Amphibia

机译：非洲爪蟾：两栖动物的理想实验动物。

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摘要

Studies using amphibians have contributed to the progress of life science including developmental biology and cell biology for more than one hundred years. Since the 1950s Xenopus laevis in particular has been used by scientists in many fields for experiments, resulting in the development of various techniques such as microsurgery on early embryos, biosynthesis of gene-encoded protein in oocytes by mRNA injection, misexpression experiments by mRNA injection into embryos, gene knockdown studies by injection of morpholino anti-sense oligonucleotide into fertilized eggs, transgenesis by the I-Scel meganuclease method, and so on. In this paper we will introduce Xenopus tropicalis as an alternative experimental animal. It has a shorter generation time and smaller diploid genome, together with whole-genome sequence data. The procedures available for Xenopus laevis can work well with Xenopus tropicalis, and embryos of both species develop at similar rates according to the developmental staging system of Nieuwkoop and Faber. Experimental systems of Xenopus tropicalis will pave the way for a new era of vertebrate genomics and genetics.
机译：使用两栖动物的研究为生命科学的进步做出了贡献，包括发育生物学和细胞生物学已有一百多年的历史。自1950年代以来，特别是非洲爪蟾被许多领域的科学家用于实验，从而导致了各种技术的发展，例如早期胚胎的显微外科手术，通过mRNA注射在卵母细胞中生物合成基因编码的蛋白质，通过将mRNA注射到卵母细胞中进行错误表达实验。胚胎，通过将吗啉代反义寡核苷酸注入受精卵中进行基因敲除研究，通过I-Scel大范围核酸酶法进行转基因等。在本文中，我们将介绍热带非洲爪蟾作为替代实验动物。它具有较短的生成时间和较小的二倍体基因组，以及全基因组序列数据。 Xenopus laevis可用的程序可以很好地与Xenopustropicis配合使用，根据Nieuwkoop和Faber的发育分期系统，两种物种的胚以相似的速率发育。热带非洲爪蟾的实验系统将为脊椎动物基因组学和遗传学的新时代铺平道路。

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  • 来源
    《Experimental Animals》 |2010年第4期|P.395-405|共11页
  • 作者

    Keiko KASHIWAGI; Akihiko KASHIWAGI; Atsushi KURABAYASHI; Hideki HANADA; Keisuke NAKAJIMA; Morihiro OKADA; Minoru TAKASE; Yoshio YAOITA;

  • 作者单位

    Division of Embryology and Genetics, Institute for Amphibian Biology, Graduate School of Science, Hiroshima University, Higashihiroshima 739-8526, Japan;

    Division of Embryology and Genetics, Institute for Amphibian Biology, Graduate School of Science, Hiroshima University, Higashihiroshima 739-8526, Japan;

    Division of Diversification Mechanism, Institute for Amphibian Biology, Graduate School of Science, Hiroshima University, Higashihiroshima 739-8526, Japan;

    Division of Embryology and Genetics, Institute for Amphibian Biology, Graduate School of Science, Hiroshima University, Higashihiroshima 739-8526, Japan;

    Division of Embryology and Genetics, Institute for Amphibian Biology, Graduate School of Science, Hiroshima University, Higashihiroshima 739-8526, Japan;

    Division of Embryology and Genetics, Institute for Amphibian Biology, Graduate School of Science, Hiroshima University, Higashihiroshima 739-8526, Japan;

    Division of Embryology and Genetics, Institute for Amphibian Biology, Graduate School of Science, Hiroshima University, Higashihiroshima 739-8526, Japan;

    Division of Embryology and Genetics, Institute for Amphibian Biology, Graduate School of Science, HiroshimaUniversity, Higashihiroshima 739-8526, Japan;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    xenopus genetic divergence; xenopus laevis; xenopus tropicalis (silurana tropicalis) laboratory lines;

    机译：爪蟾的遗传差异;非洲爪蟾热带非洲爪蟾（siluranatropicis）实验室线;

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