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首页> 外文期刊>Experimental Animals >Mitochondrial DNA Diversity among Three Subpopulations of Cynomolgus Macaques (Macaca fascicularis) Originating from the Indochinese Region
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Mitochondrial DNA Diversity among Three Subpopulations of Cynomolgus Macaques (Macaca fascicularis) Originating from the Indochinese Region

机译：源自印度支那地区的食蟹猕猴（Macaca fascicularis）的三个亚群中线粒体DNA多样性

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摘要

The cynomolgus macaque (Macaca fascicularis) has emerged as an important experimental animal model for biomedical research in various domains, necessitating the more extensive characterization of the genetic backgrounds influencing the macaque's response to drugs and sensitivity to experimental disease. The diversity of the variable mitochondrial DNA (mtDNA) D-loop region has been analyzed phylogenetically among geographically isolated populations or within subdivisions of the same regional population. However, the genetic differences among several substructures originating from a common population have not yet been investigated. By sequencing fragments of the mtDNA D-loop region from two subpopulations from the Indochinese region (Cambodian-Chinese and Vietnamese) along with two native Indonesian and Filipino populations, we identified 87 mtDNA D-loop haplotypes, of which 67 are new. The phylogenetic relationship suggests that the Indochinese haplotypes are intermingled in comparison to the distinct divergence of the Indonesian and Filipino lineages. The subpopulations were shown by estimation of evolutionary divergence and Wright's F-statistic (Fst) to have little genetic differentiation. Altogether, the subpopulations may be used in biomedical research, even though a slight difference is observed in haplotype frequencies among them. Therefore, genetic diversity analyses will be necessary for the elucidation of genetic differences among the populations, as well as to obtain a better understanding of genetic diversity for biomedical research. This will involve the selection of macaques and the monitoring of genetic heterogeneity among and within breeding facilities.
机译：食蟹猕猴（Macaca fascicularis）已成为在各个领域进行生物医学研究的重要实验动物模型，这就需要更广泛地表征影响猕猴对药物反应和对实验疾病敏感性的遗传背景。线粒体可变DNA（mtDNA）D环区域的多样性已在地理上分离的种群内或同一区域种群的细分内进行了系统发育分析。但是，尚未研究源自共同种群的几个亚结构之间的遗传差异。通过对来自印度支那地区（柬埔寨-中国人和越南人）的两个亚群的mtDNA D-loop区片段以及两个印度尼西亚和菲律宾本地居民进行测序，我们确定了87个mtDNA D-loop单倍型。系统发育关系表明，与印度尼西亚和菲律宾血统的明显差异相比，印度支那的单倍型是混杂的。通过估计进化差异和莱特的F统计量（Fst）可以显示这些亚群几乎没有遗传分化。总体而言，这些亚群可用于生物医学研究，即使它们之间的单倍型频率也有微小差异。因此，遗传多样性分析对于阐明人群之间的遗传差异以及对生物医学研究的遗传多样性有更好的了解将是必要的。这将涉及猕猴的选择以及繁殖设施之间和内部的遗传异质性监测。

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  • 来源
    《Experimental Animals》 |2010年第5期|p.567-578|共12页
  • 作者

    Takashi SHIINA; rnKeiko TANAKA; rnYoshihiko KATSUYAMA; rnKouji OTABE; rnKengo SAKAMOTO; rnMasaaki KURATA; rnMamoru NOMURA; rnHisashi YAMANAKA; rnHiroshi NAKAGAWA; rnHidetoshi INOKO; rnMasao OTA;

  • 作者单位

    Department of Molecular Life Science, Tokai University School of Medicine, Isehara, Kanagawa 259-1143 Shinshu University School of Medicine, Matsumoto, Nagano 390-8621, Japan;

    rnDepartment of Molecular Life Science, Tokai University School of Medicine, Isehara, Kanagawa 259-1143 Shinshu University School of Medicine, Matsumoto, Nagano 390-8621, Japan;

    rnDepartment of Pharmacy, Shinshu University Hospital, Matsumoto, Nagano 390-8621 Shinshu University School of Medicine, Matsumoto, Nagano 390-8621, Japan;

    rnIna Research Inc., Ina, Nagano 2148-188 Shinshu University School of Medicine, Matsumoto, Nagano 390-8621, Japan;

    rnIna Research Inc., Ina, Nagano 2148-188 Shinshu University School of Medicine, Matsumoto, Nagano 390-8621, Japan;

    rnIna Research Inc., Ina, Nagano 2148-188 Shinshu University School of Medicine, Matsumoto, Nagano 390-8621, Japan;

    rnIna Research Inc., Ina, Nagano 2148-188 Shinshu University School of Medicine, Matsumoto, Nagano 390-8621, Japan;

    rnIna Research Inc., Ina, Nagano 2148-188 Shinshu University School of Medicine, Matsumoto, Nagano 390-8621, Japan;

    rnIna Research Inc., Ina, Nagano 2148-188 Shinshu University School of Medicine, Matsumoto, Nagano 390-8621, Japan;

    rnDepartment of Molecular Life Science, Tokai University School of Medicine, Isehara, Kanagawa 259-1143 Shinshu University School of Medicine, Matsumoto, Nagano 390-8621, Japan;

    rnDepartment of Legal Medicine, Shinshu University School of Medicine, Matsumoto, Nagano 390-8621, Japan;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    cynomolgus macaques; genetic diversity; mtDNA; phylogenetic relationship; population structure;

    机译：食蟹猕猴;遗传多样性;线粒体系统发育关系人口结构;

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