• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Experimental Animals >Detection of the Antibody to Lymphocytic Choriomeningitis Virus in Sera of Laboratory Rodents Infected with Viruses of Laboratory and Newly Isolated Strains by ELISA Using Purified Recombinant Nucleoprotein
【24h】

Detection of the Antibody to Lymphocytic Choriomeningitis Virus in Sera of Laboratory Rodents Infected with Viruses of Laboratory and Newly Isolated Strains by ELISA Using Purified Recombinant Nucleoprotein

机译：纯化重组核蛋白酶联免疫吸附测定（ELISA）法检测感染了实验室和新分离株的实验室啮齿动物血清中的淋巴细胞性脑膜炎病毒抗体

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

An enzyme-linked immunosorbent assay (ELISA) was developed to detect the antibody against lymphocytic choriomeningitis virus (LCMV) in sera of laboratory animals. In this ELISA system, LCMV-nucleoprotein (NP) expressed by recombinant baculovirus and purified with high molar urea was used as the antigen. Sera from laboratory animals experimentally infected with the Armstrong strain or the newly isolated Ml strain of LCMV were examined to detect anti-LCMV antibody by the ELISA system, and the reactivity was compared with that of IFA test. Regardless of LCMV strain, all the sera of adult mice infected with LCMV were positive with very high optical density (OD). Also, the sera from mice neonatally infected with LCMV M1 strain were positive with slightly lower OD than adult mice. In contrast, all the sera of uninfected mice were negative to LCMV-NP antigen. Similarly, anti-LCMV antibodies were detected in all the sera of hamsters, mastomyses, and gerbils infected with the LCMV Armstrong strain. The results of the ELISA were in complete agreement with those of IFA, and indicate the high sensitivity and specificity of the ELISA system in the detection of anti-LCMV antibody. Because this ELISA system does not require handling infectious LCMV in the course of the antigen preparation and serological assay, there is no risk of contamination in the laboratory or nearby animal facility. In addition, by using negative control antigen in parallel with positive antigen in ELISA, we can exactly check the LCMV contamination in laboratory animals.
机译：开发了一种酶联免疫吸附试验（ELISA），以检测实验室动物血清中的淋巴细胞性脉络膜脑膜炎病毒（LCMV）抗体。在该ELISA系统中，将重组杆状病毒表达并用高摩尔尿素纯化的LCMV-核蛋白（NP）用作抗原。通过ELISA系统检查来自实验感染了阿姆斯特朗菌株或新分离的LCMV M1菌株的实验动物的血清，以检测抗LCMV抗体，并将其反应性与IFA测试相比较。无论LCMV株如何，感染LCMV的成年小鼠的所有血清均为阳性，并且具有很高的光密度（OD）。另外，新生小鼠感染LCMV M1株的血清呈阳性，OD值略低于成年小鼠。相反，未感染小鼠的所有血清对LCMV-NP抗原均为阴性。同样，在感染了LCMV阿姆斯特朗菌株的仓鼠，肥大肌和沙鼠的所有血清中都检测到了抗LCMV抗体。 ELISA结果与IFA完全一致，表明ELISA系统在检测LCMV抗体中具有很高的灵敏度和特异性。因为此ELISA系统不需要在抗原制备和血清学检测过程中处理传染性LCMV，所以在实验室或附近的动物设施中没有被污染的风险。此外，通过在ELISA中同时使用阴性对照抗原和阳性抗原，我们可以准确地检查实验动物中的LCMV污染。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号