Comparison of blood chemistry values in rats from three different measuring devices

Toshie YUSA; Kiyoaki KATAHIRA; Yuko KANAZAWA; Mitsuru SUGIHARA

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Comparison of blood chemistry values in rats from three different measuring devices

机译：三种不同测量设备的大鼠血液化学值的比较

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The purpose of this study is a comparison of blood chemistry values in rats. Eleven female and ten male rats (Jcl:Wistar) aged 9～11 weeks were used in this study. Each animal was freely provided with commercially pellet diet (CE-2, Clea Japan Co., Ltd) and with tap water. Animals were fasted overnight and then blood was taken from inferior vena cava under sodium pentobarbital anesthesia (50mg/kg). For the samples, plasma and serum from the same animal were measured with the automatic analyzers. Serum was used as sample for the measurement of TCHO, TG and BUN, and then plasma for measuring GLU, GOT and GPT. Blood chemical values examined with an automatic analyzer (Model 7180, Hitachi High-Technologies Co.) and two dry-chemistry systems (FDC 7000, and FDC 3500s, Fujifilm medical Co.,Ltd.). The overall result in the chemistry values in rats was almost compatible. Correlation coefficients were over 0.90. Equipment for clinical biochemistry determinations has been developed and even trace values are detectable. However, a statistically significant difference in variance was observed among three measuring systems for GOT, GPT and TCHO parameters. It is important to calibrate measuring system precisely with using a control sample or serum for laboratory rats.

机译：这项研究的目的是比较大鼠的血液化学值。使用11只雌性和10只雄性大鼠（Jcl：Wistar），年龄9〜11周。免费为每只动物提供商业颗粒饲料（CE-2，日本Clea Japan Co.，Ltd）和自来水。将动物禁食过夜，然后在戊巴比妥钠麻醉下（50mg / kg）从下腔静脉采血。对于样品，使用自动分析仪测量同一动物的血浆和血清。血清用作测量TCHO，TG和BUN的样品，然后血浆用于测量GLU，GOT和GPT。用自动分析仪（日立高新技术公司的7180型）和两个干化学系统（Fujifilm medical Co.，Ltd。的FDC 7000和FDC 3500s）检查血液化学值。在大鼠中化学值的总体结果几乎是相容的。相关系数超过0.90。已经开发出用于临床生化测定的设备，甚至可以检测到痕量值。但是，在三个测量系统中，GOT，GPT和TCHO参数的方差在统计学上有显着差异。使用实验室大鼠的对照样品或血清精确校准测量系统非常重要。

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《Experimental Animals》 |2008年第3supa期|p.141|共1页
作者
Toshie YUSA; Kiyoaki KATAHIRA; Yuko KANAZAWA; Mitsuru SUGIHARA;
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作者单位

Experimental Animal Center, Fukushima Medical University;

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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类动物学;
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机译：通过顺序多通道分析仪计算机（smaC-20）分析实验动物的正常血液化学值。

Comparison of blood chemistry values in rats from three different measuring devices

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