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Effects of vagotomy on the pica behavior induced by cyclophosphamide and X-ray irradiation in adult male rats

机译：迷走神经切断对成年雄性大鼠环磷酰胺和X射线照射异食癖行为的影响。

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Pica is the behavior showing the consumption of non-nutritive substances such as kaolin. It can be induced by administration of toxins or emetic agents and is recognized as an index of vomiting or nausea in rats. We previously reported that administration of cyclophosphamide increased kaolin consumption and the content of serotonin (5-HT) in the area postrema (AP) in adult male Long Evans (LE) rats (The 54th Annual Meeting of this Association). In the present study, we examined the effects of the vagotomy on the pica induced by intraperitoneal (i.p.) administration of cyclophosphamide or X-ray irradiation. The content of 5-HT in the AP was also examined. Seven days before the each experiment, abdominal vagotomy or sham operation was conducted under ketamine/xylazine anesthesia. On the experimental day, rats received the injection of cyclophosphamide (50mg/kg) or X-ray irradiation (4 Gy), and the kaolin consumption was measured at 24 h after the treatment. Another group rats were killed by decapitation at 6 h after the treatment of cyclophosphamide or X-ray irradiation. The AP was dissected, homogenized, and centrifuged. The content of 5-HT in the supernatant was determined by HPLC with electrochemical detection. Both stimuli (cyclophosphamide and X-ray) increased the kaolin consumption and the content of 5-HT in AP in sham-operated rats. Vagotomy suppressed the pica and the increased 5-HT content in AP induced by X-ray irradiation, but it did not affect the pica and 5-HT in cyclophosphamide-treated rats. These results suggest that X-ray irradiation induces pica mainly mediated by vagal afferents, but cyclophosphamide induces the pica mediated by another mechanism including the direct action of humoral factors on the AP.
机译：Pica是一种行为，表明食用了非营养性物质（例如高岭土）。它可以通过施用毒素或催吐剂来诱导，并且被认为是大鼠呕吐或恶心的指标。我们先前曾报道，成年雄性长Evans（LE）大鼠的给药后，环磷酰胺的使用会增加高岭土的消耗和血清区域（AP）中5-羟色胺（5-HT）的含量（该协会第54届年会）。在本研究中，我们检查了迷走神经切断术对腹膜内（i.p.）给予环磷酰胺或X射线照射诱导的异食癖的影响。还检查了AP中5-HT的含量。每次实验前7天，在氯胺酮/甲苯噻嗪麻醉下进行腹部迷走神经切断术或假手术。在实验当天，给大鼠注射环磷酰胺（50mg / kg）或X射线辐射（4 Gy），并在治疗后24小时测量高岭土的消耗量。另一组大鼠在治疗环磷酰胺或X射线照射后6 h被断头处死。解剖AP，均质化并离心。通过HPLC和电化学检测来测定上清液中5-HT的含量。两种刺激（环磷酰胺和X射线）均增加了假手术大鼠的高岭土消耗量和AP中5-HT的含量。迷走神经切断术抑制了X射线辐射引起的异食癖和AP中5-HT含量的增加，但对环磷酰胺治疗的大鼠中的异食癖和5-HT没有影响。这些结果表明，X射线辐照诱导迷走神经主要由迷走神经传入介导，但环磷酰胺诱导迷走神经由另一种机制介导，包括体液因子对AP的直接作用。

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