• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Experimental Animals >ELISA using recombinant S antigen for detection of mouse hepatitis virus infection
【24h】

ELISA using recombinant S antigen for detection of mouse hepatitis virus infection

机译：使用重组S抗原的ELISA检测小鼠肝炎病毒感染

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The most common serologic tests for mouse hepatitis virus (MHV) infection are ELISA and IFA. The ELISA using whole MHV antigens has disadvantages in quality control of viral antigens and frequent false positive results. Previously, we showed that the ELISA using recombinant nucleocapsid protein of MHV (MHV-rNP) generated false negative results in some naturally infected mice. Here we report the usefulness of the ELISA using recombinant spike protein of MHV (MHV-rS). S gene of MHV-S strain was RT-PCR amplified and cloned into an expression vector pGEX-6P-1. MHV-rS was produced in E.coli and used as antigens for ELISA. The following sera were used: 1) sera from mice experimentally infected with MHV strains S, A59, JHM, Pr and Nu67,2) sera from mice naturally infected with MHV (52 samples), 3) sera from uninfected mice (40 samples confirmed negative by IFA). All antisera to 5 MHV strains showed positive reactions in MHV-rS ELISA. As for the reactivity of naturally infected mouse sera, 46 of 52 samples (88%) were positive by MHV-rS ELISA. Meanwhile, only 3 of 40 sera from uninfected mice, including 15 false positive sera assessed by whole MHV antigens, were positive by MHV-rS ELISA. The MHV-rS ELISA revealed a specific detection of MHV infection with a low rate of false positive reactions. The simultaneous measurement of anti-S and anti-NP antibodies by MHV-rS and MHV-rNP ELISA provided 98% of sensitivity, which is almost equal to that of the ELISA using whole MHV antigens.
机译：小鼠肝炎病毒（MHV）感染最常见的血清学检测是ELISA和IFA。使用完整MHV抗原的ELISA在病毒抗原质量控制和频繁的假阳性结果方面存在缺点。以前，我们显示使用MHV重组核衣壳蛋白（MHV-rNP）的ELISA在一些自然感染的小鼠中产生假阴性结果。在这里，我们报告了使用MHV（MHV-rS）重组刺突蛋白进行ELISA的有用性。 RT-PCR扩增MHV-S菌株的S基因，并将其克隆到表达载体pGEX-6P-1中。 MHV-rS在大肠杆菌中产生，并用作ELISA的抗原。使用以下血清：1）实验感染MHV株S，A59，JHM，Pr和Nu67的小鼠的血清，2）自然感染MHV的小鼠的血清（52个样品），3）未感染小鼠的血清（已确认40个样品）由IFA否定）。在MHV-rS ELISA中，所有针对5株MHV菌株的抗血清均显示阳性反应。至于自然感染的小鼠血清的反应性，通过MHV-rS ELISA法，在52个样品中有46个（88％）呈阳性。同时，来自未感染小鼠的40份血清中只有3份，包括通过完整MHV抗原评估的15例假阳性血清，通过MHV-rS ELISA呈阳性。 MHV-rS ELISA显示特异性检测MHV感染，假阳性率低。通过MHV-rS和MHV-rNP ELISA同时测量抗S和抗NP抗体可提供98％的灵敏度，几乎与使用完整MHV抗原的ELISA相等。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号