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Successful microinsemination of rabbit oocytes with round spermatids

机译：圆精子细胞成功进行了兔卵母细胞的微授精

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Although the first report for rabbit offspring by round spermatid injection (ROSI) was published in 1994 (Sofikitis et al.), the production of rabbit ROSI offspring has been considered difficult due to high chromosomal abnormality (Ogonuki et al., 2004). The present study was designed to investigate the effect of oocyte activation regimens on developmental ability of rabbit ROSI oocytes. Denuded kbs: JW rabbit oocytes were treated with 5-10 μM ionomycin for 5 min before ROSI (Group-1), after ROSI (Group-2), before and after ROSI (Group-3), and before and after ROSI followed by 1-h incubation with 5 μg/ml cycloheximide + 2 mM 6-dimethylaminopurine (Group-4). Intracytoplasmic sperm injection (ICSI) was performed using non-treated oocytes (Group-5). The activation regimens influenced the proportions of ROSI oocytes cleaving after 22 to 24 h of culture (39, 29, 51, 68 and 60% in Group-1, -2, -3, -4 and-5, respectively). Oviductal transfer of the cleaved embryos resulted in implantation signs in Group-3 (2%) and Group-4 (9%), and full-term development only in Group-4 (7%). The ICSI resulted in 60% cleavage, 4% implantation and 8% full-term development (Group-5). Thus, rabbit ROSI oocytes were capable of developing into full-term when the oocytes were activated with a combined treatment of ionomycin and cycloheximide + 6-dimethylaminopurine.
机译：尽管1994年首次发表了关于通过圆形精子注射（ROSI）进行兔后代的首次报道（Sofikitis等人），但由于高度的染色体异常，一直认为兔ROSI后代的生产很困难（Ogonuki等人，2004）。本研究旨在研究卵母细胞活化方案对兔ROSI卵母细胞发育能力的影响。裸露的kbs：在ROSI（第1组）之前，ROSI（第2组）之后，ROSI（第3组）之前和之后，ROSI之前和之后，分别用5-10μM离子霉素处理JW兔卵母细胞5分钟。与5μg/ ml环己酰亚胺+ 2 mM 6-二甲基氨基嘌呤（第4组）孵育1小时。使用未经处理的卵母细胞（第5组）进行胞浆内精子注射（ICSI）。激活方案影响培养22至24小时后ROSI卵母细胞裂解的比例（第1，-2，-3，-4和-5组分别为39％，29％，51％，68％和60％）。卵裂卵的输卵管转移导致第3组（2％）和第4组（9％）的着床迹象，仅第4组的足月发育（7％）。 ICSI导致60％的卵裂，4％的植入和8％的足月发育（第5组）。因此，当用离子霉素和环己酰亚胺+ 6-二甲基氨基嘌呤联合处理激活卵母细胞时，兔ROSI卵母细胞能够发育成足月。

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