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首页> 外文期刊>Experimental Animals >Establishment of non-invasive embryo collection in Common Marmoset
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Establishment of non-invasive embryo collection in Common Marmoset

机译：Common猴非侵入性胚胎的建立

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Common Marmoset (Callithrix jacchus) embryo collection has been performed by surgical uterine flushing. Since surgical embryo collection is an invasive procedure for animals, establishment of an alternative non-invasive embryo collection technique is desirable. In this study, a non- invasive technique for the recovery of uterine stage pre-implantation embryos was developed for the common marmoset. Fifteen female marmosets older than 2 years of age were used in this study. To control ovarian cycles, 0.75μg/head of PGF2 α was administered and serum concentration of progesterone on days -1, 1,9, 11 and 13 was measured by EIA to estimate ovulation. A glass tube connected to a vacuum blood collection test tube was inserted into the vagina to collect flushing medium and uterus was percutaneously held by hand. Medium flushing was performed using a 23 G needle percutaneously inserted in the uterus. Effluent flushing medium was collected in vacuum blood collection test tube.
机译：普通Mar猴（Callithrix jacchus）的胚胎采集是通过手术冲洗子宫来进行的。由于外科手术胚胎收集是动物的一种侵入性程序，因此需要建立替代的非侵入性胚胎收集技术。在这项研究中，为普通mar猴开发了一种无创技术，用于恢复子宫期植入前的胚胎。本研究使用了15个2岁以上的雌性mar猴。为了控制卵巢周期，每头给予0.75μgPGF2α，并通过EIA测定第-1、1、9、11和13天的孕酮血清浓度以估计排卵。将连接至真空采血管的玻璃管插入阴道以收集冲洗介质，并用手经皮保持子宫。使用经皮插入子宫的23 G针进行中等冲洗。将流出的冲洗介质收集在真空采血管中。

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