The duties of the manipulating mouse embryo in Institute for Animal Experimentation Tohoku University Graduate School of Medicine

Keisuke Nishio; Seiichi Otake; Tadashi Okamura; Noriyuki Kasai

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The duties of the manipulating mouse embryo in Institute for Animal Experimentation Tohoku University Graduate School of Medicine

机译：东北大学大学院动物实验研究所操纵小鼠胚胎的职责

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In our institute, we started mouse embryo cryopreservation in 2004, microbiological cleaning and recovery of cryopreserved embryos in 2005 as a duty. We report the requests and the results of these duties from 2004 to now. Mouse embryo cryopreservation was carried out according to simple vitrification procedure. To examine a viability of cryopreserved embryos, some cryopreserved embryos were recovered and developed. When over 200 embryos were cryopereserved and over 30% of cryopreserved embryos were developed, we finished embryo cryopreservation. To clean up the microbes, 2-cell stage embryo transplanted to the recipient mice. After the transplantation the recipient mice were housed in isolator. After weaning the microbes in the recipient mice were monitored. And if the recipient mice were free from the microbes, we finished microbiological cleaning. Recovery of cryopreservated embryos was carried out as usual. Because embryo cryopreservation could be finished in 24 lines of mice, we have no problem in our cryopreservation method. But we needed to examine the viability of cryopreserved embryos again in the 11 lines therefore we have some problems in our examination techniques. And we have no problem in microbiological cleaning and recovery of cryopreserved embryos in our institute. From these results, we can state that our duties of the manipulating mouse embryo are smoothly carried out.

机译：在我们的研究所，我们从2004年开始进行小鼠胚胎的冷冻保存，并于2005年开始了微生物清洗和冷冻保存的胚胎的回收工作。我们报告了2004年至今的要求和这些职责的结果。根据简单的玻璃化程序进行小鼠胚胎冷冻保存。为了检查冷冻保存的胚胎的存活力，回收并发育了一些冷冻保存的胚胎。当对200多个胚胎进行冷冻保存，并开发了30％以上的冷冻保存胚胎时，我们便完成了胚胎冷冻保存。为了清理微生物，将2细胞期胚胎移植到受体小鼠中。移植后，将受体小鼠置于隔离器中。断奶后，监测受体小鼠中的微生物。如果受体小鼠没有微生物，我们就可以完成微生物清洗。照常进行冷冻保存的胚胎的恢复。由于胚胎冷冻保存可以在24系小鼠中完成，因此冷冻保存方法没有问题。但是我们需要在11条细胞系中再次检查冷冻保存的胚胎的生存力，因此我们的检查技术存在一些问题。而且我们研究所在微生物清洗和冷冻保存的胚胎的回收方面没有问题。从这些结果可以看出，操纵小鼠胚胎的职责得到了顺利执行。

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来源
《Experimental Animals》 |2008年第3supa期|p.155|共1页
作者
Keisuke Nishio; Seiichi Otake; Tadashi Okamura; Noriyuki Kasai;
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作者单位

Institute for Animal Experimentation Tohoku University Graduate School of Medicine;

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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类动物学;
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The duties of the manipulating mouse embryo in Institute for Animal Experimentation Tohoku University Graduate School of Medicine

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