A Genetic Quality Testing System for Early Stage Embryos in the Mouse

Hideki KATOH; Koji ODA; Kyoji HIOKI; Kaori MUGURUMA

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A Genetic Quality Testing System for Early Stage Embryos in the Mouse

机译：小鼠早期胚胎的遗传质量检测系统

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We have established a genetic quality testing system for early stage embryos of the mouse. A method of preparation of template DNA for PCR was established using the lysis buffer (1 x PCR reaction buffer supplemented with proteinase K at a concentration of 40 ug/ml) developed by the authors. We demonstrated that two 8-cell embryos of an inbred strain provide sufficient volumes of template DNA for PCR to identify the strain of embryos using four microsatellite markers (D3Mit54, D5Mit18, D6Mit15 and D8Mit50) differentiating 13 inbred strains of mice. This system will be useful in embryo banks that have recently been established worldwide for demonstrating the genetic accuracy of a given strain prior to recovery of live animals.

机译：我们已经为小鼠的早期胚胎建立了基因质量测试系统。作者开发的裂解缓冲液（1 x PCR反应缓冲液，添加了浓度为40 ug / ml的蛋白酶K）建立了用于PCR的模板DNA的制备方法。我们证明了一个自交系的两个8细胞胚胎提供了足够的模板DNA量，可用于PCR鉴定使用四个微卫星标记（D3Mit54，D5Mit18，D6Mit15和D8Mit50）区分13个小鼠自交系的胚胎。该系统将在最近在世界范围内建立的胚胎库中有用，以证明在恢复活体动物之前给定菌株的遗传准确性。

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来源
《Experimental Animals》 |2003年第5期|p.397-400|共4页
作者
Hideki KATOH; Koji ODA; Kyoji HIOKI; Kaori MUGURUMA;
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作者单位

Institute for Experimental Animals, Hamamatsu University School of Medicine, 1-20-1 Handayama, Hamamatsu, Shizuoka 431-3192;

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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类动物学;
关键词
early embryos; embryo bank; genetic quality; microsatellite markers; mouse;

机译：早期胚胎;胚胎库;遗传质量;微卫星标记;小鼠;

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机译：遗传小鼠胚胎测定法：通过功能性生物测定法改善辅助生殖技术（ART）的性能和质量测试
2. Mouse strain and quality control testing: Improved sensitivity of the mouse embryo assay with embryos from outbred mice [J] . KhanZ., WolffH.S., FredricksonJ.R., Fertility and Sterility: Official Journal of the American Fertility Society, Pacific Coast Fertility Society, and the Canadian Fertility and Andrology Society . 2013,第3期

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3. Morphology, topology and dimensions of the heart and arteries of genetically normal and mutant mouse embryos at stages S21-S23 [J] . Geyer Stefan H., Reissig Lukas F., Huesemann Markus, Journal of Anatomy . 2017,第4期

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5. Genetic analysis of roles of giantin and axin2 in development of the mouse embryo. [D] . Qian, Lihui. 2010

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6. Genetic mouse embryo assay: improving performance and quality testing for assisted reproductive technology (ART) with a functional bioassay [O] . Rebecca S. Gilbert, Brandy Nunez, Kumi Sakurai, 2016

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7. A Genetic Quality Testing System for Early Stage Embryos in the Mouse [O] . Katoh, Hideki, Oda, Koji, Hioki, Kyoji, 2003

机译：小鼠早期胚胎的遗传质量检测系统
8. Sensitivity of Vertebrate Embryos to Heavy Metals as a Criterion of Water Quality. Phase II. Bioassay Procedures Using Developmental Stages as Test Organisms. [R] . birge,wesley j. just,john j. 1975

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A Genetic Quality Testing System for Early Stage Embryos in the Mouse

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