Gene Transfer into Circulating Primordial Germ Cells of Quail Embryos

Tamao ONO; Shin-ichi MUTO; Takashi MATSUMOTO; Makoto MOCHII; Goro EGUCHI

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Gene Transfer into Circulating Primordial Germ Cells of Quail Embryos

机译：基因转移到鹌鹑胚胎的循环原始生殖细胞中

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During early stages in avian embryogenesis primordial germ cells (PGCs) show a unique migration pathway toward the gonadal anlage through the circulation. In the present study, liposomes consisting of plasmid DNA (pMiwZ; containing lacZ as a reporter) and Lipofectin were injected into the marginal veins of quail embryos during the stages PGCs were circulating in the blood vessels. The lacZ expression was then histochemically detected in the gonads at later embryonic stages, indicating the expression of the injected DNA in PGCs.

机译：在禽类胚胎发生的早期阶段，原始生殖细胞（PGCs）通过循环向性腺发育显示出独特的迁移途径。在本研究中，在PGC在血管中循环的阶段，将由质粒DNA（pMiwZ；含有lacZ作为报告基因）和脂质转染蛋白组成的脂质体注射到鹌鹑胚胎的边缘静脉中。然后在以后的胚胎阶段在性腺中组织化学检测到lacZ的表达，这表明注射的DNA在PGC中的表达。

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来源
《Experimental Animals》 |1995年第4期|p.275-278|共4页
作者
Tamao ONO; Shin-ichi MUTO; Takashi MATSUMOTO; Makoto MOCHII; Goro EGUCHI;
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作者单位

Laboratory of Developmental Biology, Faculty of Agriculture, Shinshu University, Ina 399-45, Japan;

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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类动物学;
关键词
lacZ; lipofection; pMiwZ; primordial germ cells; quail;

机译：lacZ;脂质体转染;pMiwZ;原始生殖细胞;鹌鹑;

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专利

1. Population of circulating primordial germ cells in early Japanese quail embryos [J] . Hai-Chang Li, Kanji Matsui, Tamao Ono 日本家禽学会誌 . 2001,第2期

机译：日本鹌鹑早期胚胎中循环原始生殖细胞的种群
2. Migratory Ability of Chick Primordial Germ Cells Transferred into Quail Embryos [J] . Ishiguro Susumu, Minematsu Takeo, Naito Mitsuru, The Journal of Reproduction and Development . 2009,第2期

机译：小鸡原始生殖细胞迁移到鹌鹑胚中的迁移能力。
3. Production of quail (Coturnix japonica) germline chimeras by transfer of gonadal primordial germ cells into recipient embryos [J] . Kim MA, Park TS, Kim JN, Theriogenology . 2005,第3期

机译：通过将性腺原始生殖细胞转移到受体胚胎中来生产鹌鹑（Coturnix japonica）种系嵌合体
4. In vivo gene transfer to chicken via blastodermal cells of early developmental embryos [C] . S. INADA, M. HATTORI, N. FUJIHARA Joint International Meeting of the Japanese Association for Animal Cell Technolog and and European Society for Animal Cell Technology . 1999

机译：通过早期发育胚胎的胚泡细胞转移到鸡的体内基因转移
5. Primordial Germ Cell Differentiation in Vitro: A Model for Understanding Epigenetic Reprogramming and Genome-Wide DNA Demethylation in Mouse Primordial Germ Cells. [D] . Vincent, John James. 2013

机译：原始生殖细胞的体外分化：一种模型，用于了解小鼠原始生殖细胞中的表观遗传重编程和全基因组DNA去甲基化。
6. Concentration and total number of circulating primordial germ cells in Green-legged Partridgelike chicken embryos [O] . Agata Szczerba, Takashi Kuwana, Marek Bednarczyk 2021

机译：绿腿鹧e鸡胚中循环原始生殖细胞浓度和总数
7. Intense Expression of GFP Gene in Gonads of Chicken Embryos by Transfecting Circulating Primordial Germ Cells in vitro and in vivo [O] . Mitsuru Naito, Takeo Minematsu, Takashi Harumi, 2007

机译：通过转染循环原始生殖细胞在体内循环原始生殖细胞中的循环原始生殖细胞增长的强烈表达。

Gene Transfer into Circulating Primordial Germ Cells of Quail Embryos

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