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首页> 外文期刊>Experimental Brain Research >Glial reactions in a rodent cauda equina injury and repair model
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Glial reactions in a rodent cauda equina injury and repair model

机译：啮齿类马尾马损伤和修复模型中的神经胶质反应

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In the adult rat, an avulsion injury of lumbosacral ventral roots results in a progressive and pronounced loss of the axotomized motoneurons. A subsequent acute implantation of an avulsed ventral root into the spinal cord has neuroprotective effects. However, it has not been known whether a surgical implantation of an avulsed ventral root into the spinal cord for neural repair purposes affects intramedullary glial and microglial reactions. Here, adult female Sprague-Dawley rats underwent a unilateral L5-S2 ventral root avulsion injury with or without acute implantation of the L6 ventral root into the spinal cord. At 4 weeks postoperatively, immunohistochemistry using primary antibodies to GFAP (astrocytes), Ox-42 (microglia), and ED-1 (macrophages) was performed at the L6 spinal cord segment, and quantified using densitometry. Our results show that a lumbosacral ventral root avulsion injury induces an activation of astrocytes, microglia, and macrophages in the ventral horn. Interestingly, an acute implantation of an avulsed root into the white matter does not significantly affect the activation of glial cells or macrophages in the ventral horn. We speculate that neuroprotective and axonal growth promoting benefits of the combined glial and microglial/ macrophage responses may outweigh their potential negative effects, as previous studies have shown that implantation of avulsed roots is a successful strategy in promoting reinnervation of peripheral targets.
机译：在成年大鼠中，腰ac腹根部撕脱性损伤导致轴突化运动神经元的进行性明显丧失。随后将撕脱的腹侧根部急性植入脊髓中具有神经保护作用。然而，尚不知道为神经修复的目的将撕脱的腹侧根根植入脊髓中是否会影响髓内神经胶质和小胶质细胞反应。在这里，成年雌性Sprague-Dawley大鼠遭受了单侧L5-S2腹侧根部撕脱伤，无论是否将L6腹侧根部急性植入到脊髓中。术后4周，在L6脊髓节段使用针对GFAP（星形细胞），Ox-42（小胶质细胞）和ED-1（巨噬细胞）的一抗进行免疫组织化学，并使用光密度法定量。我们的结果表明，腰s部腹根部撕脱伤可引起腹角中星形胶质细胞，小胶质细胞和巨噬细胞的活化。有趣的是，将撕脱的根急性植入白质中不会显着影响腹角中神经胶质细胞或巨噬细胞的活化。我们推测神经胶质和小胶质细胞/巨噬细胞反应的组合在神经保护和轴突生长方面的益处可能超过其潜在的负面影响，因为以前的研究表明，撕脱根部植入是促进周围靶标神经支配的成功策略。

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