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Neurogenesis of cuneothalamic neurons and NO-containing neurons in the cuneate nucleus of the rat

机译：大鼠楔形核中丘脑丘脑神经元和含NO神经元的神经发生

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摘要

The genesis of the cuneothalamic neurons (CTNs) in the rat cuneate nucleus was determined by a double-labeling method using 5′-bromodeoxyuridine (BrdU), the thymidine analogue, and Fluoro-Gold (FG), a retrograde fluorescent tracer. BrdU-positive cells were observed in the cuneate nucleus in all rats receiving BrdU injection at embryonic days (E) E13–E16; none was detected in rats given BrdU injection at E12. At E13 and E14, BrdU-positive cells were randomly distributed. However, at E15, the number of BrdU-positive cells was clearly reduced and the majority of them was located at the dorsolateral or peripheral region of the nucleus. FG/BrdU double-labeling study showed the existence of BrdU-labeled CTNs when the mother rat received BrdU injection at E13 and E14, being more numerous at E13 in which the neurons were scattered throughout the nucleus. At E14, however, the majority of the BrdU-labeled CTNs were located superficially in the nucleus. Double-labeled cells were undetected in rats that had been exposed to BrdU at E15 and E16. Quantitative data showed that the majority (ca 70–80%) of the CTNs were generated at E13, and were markedly decreased at E14 (ca 4–6%). Using nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) histochemistry coupled with BrdU immunohistochemistry, we have shown the NADPH-d/BrdU double-labeled neurons in the nucleus between E13 and E15, with the majority of them occurring at E14, but absent at E16. The present results suggest that the CTNs are generated prior to the NO-containing neurons in the cuneate nucleus.
机译：通过双标记方法使用5'-溴脱氧尿苷（BrdU），胸苷类似物和逆行荧光示踪剂Fluor-Gold（FG）通过双标记方法确定大鼠楔形核中丘脑丘脑神经元（CTN）的起源。在胚胎天（E）E13–E16，所有接受BrdU注射的大鼠的楔形核中均观察到BrdU阳性细胞；在E12注射BrdU的大鼠中均未检测到。在E13和E14，BrdU阳性细胞随机分布。但是，在E15时，BrdU阳性细胞的数量明显减少，并且大多数位于核的背外侧或外围区域。 FG / BrdU双重标记研究表明，当母鼠在E13和E14接受BrdU注射时，存在BrdU标记的CTN，在E13时神经元散布在整个细胞核中。但是，在E14时，大多数BrdU标记的CTN位于表面核中。在E15和E16暴露于BrdU的大鼠中未检测到双标记细胞。定量数据显示，大多数CTN（约70–80％）在E13产生，在E14时显着下降（约4–6％）。通过使用烟酰胺腺嘌呤二核苷酸磷酸重氮酶（NADPH-d）的组织化学结合BrdU免疫组织化学，我们显示了NADPH-d / BrdU双标记神经元在E13和E15之间的细胞核中，大多数发生在E14，但不存在在E16。目前的结果表明，CTN在楔形核中的含NO神经元之前产生。

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  • 来源
    《Experimental Brain Research》 |2002年第3期|327-334|共8页
  • 作者

    Tsyr-Jiuan Wang; June-Horng Lue; Ching-Hsiang Wu; Jeng-Yung Shieh; Chen-Yuan Wen;

  • 作者单位

    Department of Anatomy and Cell Biology College of Medicine National Taiwan University 1 Sec. 1 Jen-Ai Road Taipei Taiwan;

    Department of Anatomy and Cell Biology College of Medicine National Taiwan University 1 Sec. 1 Jen-Ai Road Taipei Taiwan;

    Department of Biology and Anatomy National Defense Medical Center 161 Sec. 6 Min-Chaun East Road Taipei Taiwan;

    Department of Anatomy and Cell Biology College of Medicine National Taiwan University 1 Sec. 1 Jen-Ai Road Taipei Taiwan;

    Department of Anatomy and Cell Biology College of Medicine National Taiwan University 1 Sec. 1 Jen-Ai Road Taipei Taiwan;

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  • 关键词

    Neurogenesis Bromodeoxyuridine Cuneothalamic neurons NADPH-d/NOS-IR neurons Retrograde tracing;

    机译：神经发生溴脱氧尿苷丘脑丘脑神经元NADPH-d / NOS-IR神经元逆行示踪。;

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