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The Experiment Research of Subconjunctival Injections with Etoposide for the Treatment of Retinoblastoma Mice

机译：结膜下注射依托泊苷治疗视网膜母细胞瘤小鼠的实验研究

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Purpose: To evaluate the efficacy of subconjunctival injections of Etoposide for retinobl-astoma(RB) mice. Method: Twenty-five RB mice were divided into 5 groups for the study(G0～G4). G0 for control group received 0.9% sodium chloride injection subconjunctival. G1: 5 RB mice for systemic delivery received 6 systemic administration of Etoposide 150 μg and Cyclosporin A (CSA )1.0mg at 3 days intervals, respectively. G2～G4; 5 RB mice in each group received 6 subconjunctival delivery of Etoposide in 50, 75, 150 μg at 3 days intervals, respectively.To observe the changes of the volume of RB in anterior chamber and the part of conjunctiva for injections of chemotherapeutic drugs. All eyes and conjunctiva and scleras of the part for drugs injections were obtained at 6 weeks after experiment for histopathologic examination. The findings under the light microscope were analysed with software to measure the areas of RB in anterior chambers and the tumor inhibition rate. Results: The volume of RB in anterior chambers for G1 and G2 increase quickly and none of the mice exhibited tumor control. The RB volume increase slowly in G3 and exhibit tumor control in G4. The areas of tumors in G0～G4 are 0.710, 0.659, 0.634, 0.515, 0.235 mm~2, respectively. The rate for tumor inhibition in G0～G4 are 0.0%, 7.18%, 10.70%, 27.43%, 66.90%, respectively. There are statistical significant difference between the G1 and G4 for the volumes of tumors and the rate of tumor inhibition. The pathologic examination of subconjunctiva and sclera for the injective sites showed no pathologic changes. Conclusions: There are significant effects for the method of subconjunctival delivery of Etoposide in RB control, demonstrating a positive dose-related relationship. Subconjunctival injection of Etoposide reached better efficacy in controlling and inhibiting RB cells than systemic delivery of Etoposide for the same dose. The pathologic results showed the subconjunctival delivery of etoposide were safety for conjunctiva and sclera. It would be one of effective and safety method of chemotherapy for RB.
机译：目的：评估结膜下注射依托泊苷对视网膜母细胞瘤（RB）小鼠的疗效。方法：25只RB小鼠分为5组（G0〜G4）。对照组G0结膜下注射0.9％氯化钠注射液。 G1：5只用于全身递送的RB小鼠分别在3天的间隔内分别接受了6次全身性给予依托泊苷150μg和环孢菌素A（CSA）1.0mg的全身性给药。 G2〜G4；每组5只RB小鼠分别在3天的间隔分别接受50、75、150μg的依托泊苷6次结膜下分娩，观察前房和结膜部位RB的体积变化，以供注射化疗药物。在进行组织病理学检查的实验后第6周，获得了用于注射药物的部分的所有眼睛，结膜和巩膜。用软件分析光学显微镜下的发现，以测量前房中RB的面积和肿瘤抑制率。结果：G1和G2前房的RB体积迅速增加，并且没有小鼠表现出肿瘤控制能力。 RB体积在G3中缓慢增加，在G4中显示出肿瘤控制能力。 G0〜G4的肿瘤面积分别为0.710、0.659、0.634、0.515、0.235 mm〜2。 G0〜G4的抑瘤率分别为0.0％，7.18％，10.70％，27.43％，66.90％。 G1和G4之间的肿瘤体积和肿瘤抑制率之间存在统计学差异。结膜下和巩膜的注射部位的病理检查未发现病理变化。结论：依托泊苷的结膜下递送方法在RB控制中具有显着效果，表明剂量相关呈正相关。与相同剂量的依托泊苷全身给药相比，结膜下注射依托泊苷在控制和抑制RB细胞方面具有更好的疗效。病理结果表明依托泊苷的结膜下递送对结膜和巩膜安全。这将是RB化疗的一种安全有效的方法。

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