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Adenovirally Delivered Brain-derived Neurotrophic Factor to Rat Retina

机译：腺病毒向大鼠视网膜传递脑源性神经营养因子

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摘要

Purpose: To study the expression of brain-derived neurotrophic factor (BDNF) in the rat retina delivered by adenovirus. Methods: Adenovirus with BDNF gene was injected into the vitreous. Gene expression was detected by immunofluorescence staining, and quantitative analysis was performed after injury and transfection by Enzyme-linked immunosorbent assay (ELISA). Results: The positive cells can be seen on the 3ri day and last 4 weeks by immunofluorescence staining. Positive cells in the control group were fewer than those in the transfection group or the fluorescence intensity was lower at every time point. Quantitative analysis showed that the expression of BDNF groups was higher than that of the control group at every time point(P < 0.01) , and that of the injured group without transfection was higher than that of the control group on the 3rd day and the 7th day (P < 0.01). Conclusion: Efficient and stable transfer of BDNF gene could be achieved by adenovirus delivery into the retina of rats. Injury can promote the expression of BDNF in early period.
机译：目的：研究腺病毒释放的大鼠视网膜中脑源性神经营养因子（BDNF）的表达。方法：将BDNF基因的腺病毒注入玻璃体。通过免疫荧光染色检测基因表达，并在损伤和转染后通过酶联免疫吸附测定（ELISA）进行定量分析。结果：通过免疫荧光染色可以在第3天和最后4周看到阳性细胞。对照组中的阳性细胞少于转染组，或者每个时间点的荧光强度都较低。定量分析表明，BDNF各组在各时间点的表达均高于对照组（P <0.01），未转染的损伤组在第3天和第7天的表达均高于对照组。天（P <0.01）。结论：通过腺病毒向大鼠视网膜内转移可以实现BDNF基因的高效稳定转移。损伤可在早期促进BDNF的表达。

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  • 来源
    《Eye Science》 |2004年第3期|p.187-190|共4页
  • 作者

    Xu Hou; Dan Hu; Yannian Hui;

  • 作者单位

    Department of Ophthalmology, Xijing Hospital, The Fourth Military Medical University, Xi'an 710033, China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 R7;
  • 关键词

    optic nerve; BDNF; transfection;

    机译：视神经;BDNF;转染;
  • 入库时间 2022-08-18 00:50:51

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