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首页> 外文期刊>Eye Science >Inhibition on Telomerase Activity and Cytotoxic Effects by Cisplatin in Cultured Human Choroidal Melanoma Cells
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Inhibition on Telomerase Activity and Cytotoxic Effects by Cisplatin in Cultured Human Choroidal Melanoma Cells

机译：顺铂对人脉络膜黑色素瘤细胞端粒酶活性的抑制和细胞毒作用

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摘要

Purpose: To study the changes of telomerase activity and cytotoxic effects by Cisplatin; cis-dichlorodiamine platinum (CDDP) in cultured human choroidal melanoma. Material and Methods: The primary cultured human choroidal melanoma cells were cultured in the presence and absence of CDDP with different concentration and time respectively. The toxic effects were evaluated by MTT and the level of telormarse was detected by PCR-ELISA assay. And the relationship between telomerase activity and cytotoxic effects were analyzed by a correlation analysis. Results: Following the increase of the concentration and the time of CDDP, gradually repressed telomerase activity was detected in cultured cells. Meanwhile, the restrain rate of the cells increased. The telomerase activity at 24h and 1μg/ml was repressed significantly compared with the control cells. However, the appearance of cell death lagged behind the decreasing of telomerase. Conclusions: CDDP is an effective telomerase inhibitor in cultured choroidal melanoma cells of human eyes, which presents concentration and time dependency and can cause the death of cultured cells.
机译：目的：研究顺铂对端粒酶活性和细胞毒性作用的影响；培养的人脉络膜黑色素瘤中的顺二氯二胺铂（CDDP）。材料与方法：原代培养的人脉络膜黑色素瘤细胞在存在和不存在CDDP的情况下分别以不同的浓度和时间进行培养。用MTT法评价其毒性作用，用PCR-ELISA法检测telormarse的水平。并通过相关分析分析了端粒酶活性与细胞毒性作用之间的关系。结果：随着CDDP浓度和时间的增加，在培养的细胞中检测到逐渐降低的端粒酶活性。同时，细胞的抑制率增加。与对照细胞相比，在24h和1μg/ ml的端粒酶活性被显着抑制。然而，细胞死亡的出现滞后于端粒酶的减少。结论：CDDP是人眼培养的脉络膜黑色素瘤细胞中一种有效的端粒酶抑制剂，具有浓度和时间依赖性，可导致培养细胞死亡。

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