• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Eye Science >Efforts of Transgene Oncostatin M on the Development of Retinal Neuron in Transgenic Mice
【24h】

Efforts of Transgene Oncostatin M on the Development of Retinal Neuron in Transgenic Mice

机译：转基因制瘤素M对转基因小鼠视网膜神经元发育的影响

获取原文
获取原文并翻译 | 示例
       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Purpose: Oncostatin M(OSM) is a cytokine released by macrophages and lymphocytes that can function as a growth regulator. A current study shows that leukemia inhibitory factor (LIF), a homologue of OSM, can prevent photoreceptor cell death when expressed in the lens of transgenic mice. We determined the efforts of lens-specific overexpression of OSM on the development of eye. Methods: A truncated mouse OSM cDNA (～660 bp) was linked to the αA-crytallin promoter, and injected into single-cell embryos with microinjection. Then, transgenic mice were established. The mRNA expression of transgene OSM was detected by in situ hybridization. Immunohistochemistry was used to detect the expression of syntaxin, glial fibrillary acidic protein (GFAP), synaptophysin in the retinas of transgenic mice. Results: At embryonic day (E 17. 5), the expression of the syntaxin at the inner and mid portion of the retinas of transgenic mice was much higher than that of the retinas of non-transgenic mice. The expression of GFAP was detected in the retinas of transgenic mice, while no expression in non-transgenic normal FVB( FVB/N) mice was detected in this stage. At postnatal day one (P1), the expression of synaptophysin was detected in the retinas of transgenic mice, but there was no such expression in FVB/N mice. Conclusions: Lens-specific overexpression of OSM induces premature differentiation of amacrine cells, gial cells, and photoreceptors in vivo.
机译：目的：制瘤素M（OSM）是巨噬细胞和淋巴细胞释放的细胞因子，可起生长调节剂的作用。当前的研究表明，白血病抑制因子（LIF）是OSM的同系物，当在转基因小鼠的晶状体中表达时，可以防止感光细胞死亡。我们确定了OSM特定于晶状体的过度表达对眼睛发育的作用。方法：将截短的小鼠OSM cDNA（约660 bp）与αA-晶状蛋白启动子连接，并通过显微注射将其注射到单细胞胚胎中。然后，建立转基因小鼠。通过原位杂交检测转基因OSM的mRNA表达。免疫组织化学法检测转基因小鼠视网膜中syntaxin，神经胶质原纤维酸性蛋白（GFAP），突触素的表达。结果：在胚胎发育的第1天（E 17. 5），syntaxin在转基因小鼠视网膜内部和中部的表达远高于非转基因小鼠视网膜的表达。在此阶段，在转基因小鼠的视网膜中检测到GFAP的表达，而在非转基因的正常FVB（FVB / N）小鼠中未检测到GFAP的表达。在出生后第一天（P1），在转基因小鼠的视网膜中检测到突触素的表达，但在FVB / N小鼠中没有这种表达。结论：OSM的晶状体特异性过表达在体内诱导无长突细胞，胶质细胞和感光细胞的过早分化。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号