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Structural characterization of carangid fish myoglobins

机译：Carangid鱼肌球蛋白的结构表征

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摘要

The primary structures of myoglobin (Mb) from the following five carangid species were determined: yellowtail Seriola quinqueradiata, greater amberjack Seriola dumerili, yellowtail kingfish Seriola lalandi, Japanese horse mackerel Trachurus japonicus, and silver trevally Pseudocaranx dentex. The sequences were of varying composition both in the coding and in the noncoding regions, but all contained the open reading frame of 444 nucleotides encoding 147 amino acids. Amino acid sequence identities of carangid Mbs were in the range of 81–99%. The similarity of the heme pocket and associated heme-binding residues of carangid Mbs were evidence of the conservative nature of Mbs. Similar to the other teleost Mbs, carangid Mbs did not contain a D helix and had mostly conserved A and E helices as well as E–F and G–H inter-helical segments. Hydropathy profiles of carangid Mbs showed species-specific variations where silver trevally Mb exhibited generally higher hydrophobicity. Phylogenetic analysis based on the primary structures was in agreement with conventional morphological taxonomy, establishing close proximity of carangid Mbs with those of cichlid and scombroid, the other members of the Perciformes order.
机译：确定了来自以下五种类近缘动物的肌红蛋白（Mb）的主要结构：黄尾金丝雀（Seriola quinqueradiata），greater鱼更大的金丝雀（Seriola dumerili），黄尾金鱼金丝雀（Seriola lalandi），日本竹荚鱼Trachurus japonicus和银ev体假单壳an。该序列在编码区和非编码区中具有不同的组成，但是全部包含444个核苷酸的开放阅读框，其编码147个氨基酸。 Carangid Mbs的氨基酸序列同一性在81–99％的范围内。类胡萝卜素血红蛋白的血红素袋和相关的血红素结合残基的相似性证明了血红蛋白的保守性。与其他硬骨的Mb相似，类拟南芥Mb不包含D螺旋，并且大部分是保守的A和E螺旋，以及E-F和G-H螺旋间段。 Carangid Mbs的亲水性谱显示了特定于物种的变化，其中在银上Mb表现出通常更高的疏水性。基于一级结构的系统发育分析与常规形态分类学相一致，建立了类胡萝卜素类单克隆抗体与慈鲷类其他成员的丽鱼科动物和scombroid的相似性。

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  • 来源
    《Fish Physiology and Biochemistry》 |2012年第5期|p.1311-1322|共12页
  • 作者

    Muhammad Mehedi Hasan; Shugo Watabe; Yoshihiro Ochiai;

  • 作者单位

    Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo, 113-8657, Japan;

    Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo, 113-8657, Japan;

    Department of Fisheries Science, School of Marine Science and Technology, Tokai University, Shimizu, Shizuoka, 424-8610, Japan;

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  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    Myoglobin; Carangids; Fish; Primary structure; Phylogeny;

    机译：肌红蛋白;类胡萝卜素;鱼;一级结构;系统发育;

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