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首页> 外文期刊>Fish Physiology and Biochemistry >The yellow catfish, Pelteobagrus fulvidraco (Siluriformes) metallothionein cDNA: molecular cloning and transcript expression level in response to exposure to the heavy metals Cd, Cu, and Zn
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The yellow catfish, Pelteobagrus fulvidraco (Siluriformes) metallothionein cDNA: molecular cloning and transcript expression level in response to exposure to the heavy metals Cd, Cu, and Zn

机译：黄色cat鱼，Pelteobagrus fulvidraco（Siluriformes）金属硫蛋白cDNA：分子克隆和转录表达水平，响应重金属镉，铜和锌的暴露

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摘要

Metallothionein (MT) has been used extensively as a potential molecular biomarker to detect heavy metal pollution in aquatic organisms. In order to investigate the modulation effect of heavy metals and to establish suitable biomarkers for the monitoring of heavy metal pollution, Pelteobagrus fulvidraco metallothionein gene was characterized as the first report in the family Bagridae. Pf-MT transcript was detected at high levels in liver, gonad, kidney, and brain compared to other tissues. A time-course study in response to waterborne Cd (5 ppm) revealed that a significant increase in the Pf-MT transcript abundance was observed at 6 h in gill, kidney, and liver. These elevated levels were kept for 96 h, implying that Cd distributed fast into different organs and was involved in the tissue-specific induction pattern. We observed a significant Pf-MT transcript increase in liver tissues at 48 h, followed by gill at 12 h and intestine at 48 h after Cd exposure. This indicates hepatic MT expression as a potential biomarker of acute Cd exposure in this species. Cd-binding ability of recombinant Pf-MT protein provided evidence for sensitivity to Cd and other heavy metal exposure. In the case of Zn exposure (1 ppm), a significant increase in Pf-MT transcript abundance was observed at 12 h, and a peak induction level reaching sixfold at 24 h was kept until 48 h, showing similar transcript induction patterns with Cd. A high level of Pf-MT mRNA after exposure to Cu (1 ppm) was observed at 12 h that gradually increased until 96 h with a 12-fold induction, revealing a long-lasting induction and somewhat dissimilar pattern compared to other metals in liver. Our results demonstrate that Pf-MT can be induced by heavy metals in a tissue-specific and metal-specific manner and plays probably a conserved role in metal detoxification. This study provides new information on P. fulvidraco metallothionein gene for the use of biomarkers indicating metal pollution in fish.
机译：金属硫蛋白（MT）已被广泛用作检测水生生物中重金属污染的潜在分子生物标记。为了研究重金属的调节作用并建立用于监测重金属污染的合适生物标志物，将Pelteobagrus fulvidraco metallothionein基因表征为Bagridae家族的首次报道。与其他组织相比，在肝，性腺，肾脏和脑中检测到高水平的Pf-MT转录本。一项针对水性Cd（5 ppm）的时程研究表明，在g，肾脏和肝脏中，在6 h观察到Pf-MT转录本丰度显着增加。这些升高的水平保持96小时，这表明Cd快速分布到不同器官中，并参与组织特异性诱导模式。我们观察到，在暴露于Cd后48小时，肝脏组织中Pf-MT转录物显着增加，随后在12 h时出现g，在48 h时出现肠。这表明肝脏MT表达是该物种急性Cd暴露的潜在生物标记。重组Pf-MT蛋白的Cd结合能力为Cd和其他重金属暴露的敏感性提供了证据。在锌暴露（1 ppm）的情况下，在12 h时观察到Pf-MT转录物丰度显着增加，并且在24 h时达到48倍的峰值诱导水平一直保持到48 h，显示出与Cd相似的转录物诱导模式。暴露于铜（1 ppm）后，在12 h观察到高水平的Pf-MT mRNA，诱导后逐渐增加直至96 h，诱导率是12倍，与肝脏中的其他金属相比，显示出持久的诱导作用，并且模式与肝脏中的其他金属略有不同。我们的结果表明，Pf-MT可以由重金属以组织特异性和金属特异性方式诱导，并且可能在金属排毒中起保守作用。这项研究提供了有关黄腐体育金属硫蛋白基因的新信息，可用于指示鱼类中金属污染的生物标记物的使用。

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  • 来源
    《Fish Physiology and Biochemistry》 |2012年第5期|p.1331-1342|共12页
  • 作者

    Jin-Hyoung Kim; Jae-Sung Rhee; Hans-Uwe Dahms; Young-Mi Lee; Kyung-Nam Han; Jae-Seong Lee;

  • 作者单位

    National Research Lab of Marine Molecular and Environmental Bioscience, Department of Chemistry, College of Natural Sciences, Hanyang University, Seoul, 133-791, South Korea;

    Department of Molecular and Environmental Bioscience, Graduate School, Hanyang University, Seoul, 133-791, South Korea;

    Department of Green Life Science, College of Natural Sciences, Sangmyung University, Seoul, 110-743, South Korea;

    Department of Green Life Science, College of Natural Sciences, Sangmyung University, Seoul, 110-743, South Korea;

    Department of Marine Science, College of Natural Sciences, Inha University, Incheon, 402-751, South Korea;

    National Research Lab of Marine Molecular and Environmental Bioscience, Department of Chemistry, College of Natural Sciences, Hanyang University, Seoul, 133-791, South Korea;

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  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    Catfish; Pelteobagrus fulvidraco; Metallothionein; Heavy metal;

    机译：fish鱼;黄bag;金属硫蛋白;重金属;

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