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首页> 外文期刊>Fish Physiology and Biochemistry >Stability of RNA isolated from post-mortem tissues of Atlantic salmon (Salmo salar L.)
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Stability of RNA isolated from post-mortem tissues of Atlantic salmon (Salmo salar L.)

机译：从大西洋鲑（Salmo salar L.）的死后组织分离的RNA的稳定性

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Studies of post-mortem interval on the stability of RNA from a number of various mammals have shown RNA to be stable for between 24 and 48 h following death. As yet there have been no studies looking at RNA stability in post-mortem tissues of poikilothermic fish. Brain, kidney, liver and muscle were collected from Atlantic salmon (Salmo salar) parr and samples of each tissue were placed into RNAlater? after 0–24 h post-mortem storage at room temperature. Electrophoretic analysis of the total RNA showed degradation of ribosomal RNA only in muscle from 8 h onwards. Probing of northern blots with β-actin showed that, in the brain, β-actin mRNA was stable for 24 h post-mortem but degradation of mRNA was observed after 8 h with the kidney and liver and after 4 h with the muscle. Expression of the weakly expressed thyroid hormone receptor β (TRβ) was detected by reverse transcriptase polymerase chain reaction (RT-PCR) in all tissues up to 24 h post-mortem although a reduction in PCR product was observed after 8 h with muscle and 24 h with kidney. Analysis with an Agilent 2100 Bioanalyzer showed that the RNA integrity number (RIN) of brain total RNA remained constant for 8 h post-mortem with only a small fall at 24 h post-mortem. The RINs of the remaining tissues indicated degradation at 8 h post-mortem with kidney and muscle and at 24 hours post-mortem with liver. Taken together these findings show that degradation of Atlantic salmon RNA is tissue dependent but stable for at least one hour post-mortem.
机译：死后间隔对多种哺乳动物的RNA稳定性的研究表明，死亡后RNA在24至48小时内稳定。迄今为止，还没有任何研究研究在Poikilothermic鱼的死后组织中的RNA稳定性。从大西洋鲑（Salmo salar）parr收集脑，肾，肝和肌肉，并将每种组织的样品放入RNAlater？中。在室温下将尸体存放0-24小时后。总RNA的电泳分析表明，从8小时开始，仅在肌肉中降解了核糖体RNA。用β-肌动蛋白探测northern印迹显示，在脑中，β-肌动蛋白mRNA在死后24小时内稳定，但在肾脏和肝脏8 h后和肌肉4 h后观察到mRNA降解。直到死后24 h，通过逆转录聚合酶链反应（RT-PCR）在所有组织中检测到了弱表达的甲状腺激素受体β（TRβ）的表达，尽管在肌肉和24 h的肌肉中经过8 h后观察到PCR产物减少。 h有肾脏。使用Agilent 2100生物分析仪进行的分析表明，脑总RNA的RNA完整性值（RIN）在验尸后8小时保持恒定，而在验尸后24小时只有很小的下降。其余组织的RINs表明在死后8小时，肾脏和肌肉降解，在死后24小时，肝脏降解。综上所述，这些发现表明大西洋鲑鱼RNA的降解是组织依赖性的，但在死后至少一小时内稳定。

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