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首页> 外文期刊>Fish Physiology and Biochemistry >Anti-oxidant status in embryonic, post-hatch and larval stages of Asian seabass (Lates calcarifer)
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Anti-oxidant status in embryonic, post-hatch and larval stages of Asian seabass (Lates calcarifer)

机译：亚洲鲈鱼胚胎，孵化后和幼体阶段的抗氧化状态

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摘要

The concentrations of anti-oxidant enzymes such as superoxide dismutase (SOD), catalase (CAT) and selenium-dependent glutathione peroxidase (SeGPx), and low molecular weight free-radical scavengers such as reduced glutathione (GSH) and ascorbic acid (vitamin C) were evaluated during the period from gastrulation (GS) to 25 days post-hatch (dph) in the larvae of Asian Seabass, Lates calcarifer. Oxidative damage due to lipid peroxidation (LPO) was also assessed, by evaluation of the formation of malondialdehyde (MDA). All the three anti-oxidant enzymes, SOD, CAT and GPx, showed high activities during gastrulation, suggesting an increased metabolic rate during the period of embryonic development. Though the SOD activity apparently decreased progressively during 3–20 dph of larval development, the difference was not significant. CAT showed high activity during gastrulation and remained constant up to 3 dph, suggesting an increased need to metabolise hydrogen peroxide (H2O2) and organic peroxides. In contrast, SeGPx activity increased progressively from 5 dph to 25 dph during larval development, indicating an increased need to detoxify lipid peroxides. This is evident from the observation of increased lipid peroxidation from 10 dph to 25 dph during larval development. GSH levels were low at gastrulation, indicating increased metabolic rate and formation of lipid radicals during this period, corresponding to the decrease in the level of ascorbic acid, which is consumed for regeneration of GSH.
机译：诸如超氧化物歧化酶（SOD），过氧化氢酶（CAT）和硒依赖性谷胱甘肽过氧化物酶（SeGPx）等抗氧化剂的浓度，以及低分子量自由基清除剂如还原型谷胱甘肽（GSH）和抗坏血酸（维生素C ）在亚洲鲈鱼（Lates calcarifer）幼体的从气化（GS）到孵化后25天（dph）的期间内进行了评估。还通过评估丙二醛（MDA）的形成来评估由于脂质过氧化（LPO）引起的氧化损伤。所有三种抗氧化酶，SOD，CAT和GPx，在胃形成过程中均表现出高活性，表明在胚胎发育期间代谢率增加。尽管在幼体发育3-20 dph时SOD活性明显下降，但差异并不显着。 CAT在气化过程中表现出高活性，并保持恒定至3 dph，这表明代谢过氧化氢（H2 O2 ）和有机过氧化物的需求增加。相反，在幼体发育过程中，SeGPx活性从5 dph逐渐增加到25 dph，表明对脂质过氧化物的解毒需求增加。这从幼虫发育过程中脂质过氧化从10 dph增加到25 dph的观察中可以明显看出。 GSH在胃泌尿过程中的水平很低，表明在此期间代谢率增加和脂质自由基的形成，对应于抗坏血酸水平的降低，而抗坏血酸的水平被GSH的再生所消耗。

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