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首页> 外文期刊>Fish Physiology and Biochemistry >The changes in the biochemical compositions and enzymatic activities of rotifer (Brachionus plicatilis, Müller) and Artemia during the enrichment and starvation periods
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The changes in the biochemical compositions and enzymatic activities of rotifer (Brachionus plicatilis, Müller) and Artemia during the enrichment and starvation periods

机译：轮虫（Brachionus plicatilis，Müller）和卤虫在富集和饥饿时期的生化组成和酶活性的变化

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The changes in the biochemical compositions and enzymatic activities of rotifer (Brachionus plicatilis) and Artemia, enriched and stored at 4°C temperature, were determined. The total starvation period was 16 h and samples were taken at the end of the 8th and 16th hours. In present study, the rotifer and nauplii catabolized a large proportion of the protein during the enrichment period. Lipid contents of both live preys increased during the enrichment period and decreased in nauplii and metanauplii throughout the starvation period but lipid content of the rotifer remained relatively constant during the starvation period. The changes observed in the amino acid compositions of Artemia and the rotifer were statistically significant (P < 0.05). The conspicuous decline the essential amino acid (EAA) and nonessential amino acid (NEAA) content of the rotifer was observed during the enrichment period. However, the essential amino acid (EAA) and nonessential amino acid (NEAA) contents of Artemia nauplii increased during the enrichment period. The unenriched and enriched rotifers contained more monounsaturated fatty acid (MUFAs) than polyunsaturated fatty acid (PUFAs) and saturated fatty acids (SFA). However, Artemia contained more PUFAs than MUFAs and SFA during the experimental period. A sharp increase in the amounts of docosahexaenoic acid (DHA) during the enrichment of the rotifer and Artemia nauplii was observed. However, the amount of DHA throughout the starvation period decreased in Artemia metanauplii but not in Artemia nauplii. Significant differences in tryptic, leucine aminopeptidase N (LAP), and alkaline phosphatase (AP) enzyme activities of Artemia and rotifer were observed during the enrichment and starvation period (P < 0.05). The digestive enzymes derived from live food to fish larvae provided the highest contribution at the end of the enrichment period. In conclusion, the results of the study provide important contributions to determine the most suitable live food offering time for marine fish larvae. Rotifer should be offered to fish larvae at the end of the enrichment period, Artemia nauplii just after hatching and before being stored at 4°C, and Artemia metanauplii at the end of the enrichment and throughout the starvation period.
机译：测定了在4℃温度下富集和储存的轮虫（Brachionus plicatilis）和卤虫的生化组成和酶活性的变化。总饥饿时间为16小时，并在第8和16小时结束时取样。在目前的研究中，轮虫和无节幼体在富集期间分解了大部分蛋白质。整个饥饿期间，两个活体猎物的脂质含量均增加，而幼体和无节幼体中的脂质含量均下降，但轮虫的脂质含量在饥饿期间保持相对恒定。在卤虫和轮虫的氨基酸组成中观察到的变化具有统计学意义（P <0.05）。在富集期内观察到轮虫的必需氨基酸（EAA）和非必需氨基酸（NEAA）含量明显下降。然而，在富集时期，无节幼体的必需氨基酸（EAA）和非必需氨基酸（NEAA）含量增加。未富集和富集的轮虫比多不饱和脂肪酸（PUFA）和饱和脂肪酸（SFA）含有更多的单不饱和脂肪酸（MUFA）。但是，在实验期间，Artemia中的PUFA比MUFA和SFA多。在轮虫和卤虫无节幼体的富集过程中，二十二碳六烯酸（DHA）的量急剧增加。但是，整个饥饿期中的DHA量在节肢不全症中减少，而节肢不全中没有减少。在富集和饥饿期间，卤虫和轮虫的胰蛋白酶，亮氨酸氨肽酶N（LAP）和碱性磷酸酶（AP）酶活性存在显着差异（P <0.05）。在富集期结束时，从活食到鱼幼虫的消化酶贡献最大。总之，研究结果为确定海水鱼幼体最合适的活食提供时间做出了重要贡献。在富集期结束时应向幼虫提供轮虫，孵化后并在4°C下储存前应提供幼虫无节幼体，在富集结束时和整个饥饿期应提供成虫无节幼体。

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